Send to

Choose Destination
RNA Biol. 2016;13(3):320-30. doi: 10.1080/15476286.2016.1142038. Epub 2016 Jan 29.

Protein cofactor competition regulates the action of a multifunctional RNA helicase in different pathways.

Author information

a Institute for Molecular Biology, Georg-August University , Goettingen , Germany.
b Institute for Physical Chemistry, University of Muenster , Muenster , Germany.
c Max-Planck-Institute for Biophysical Chemistry , Goettingen , Germany.
d III. Institute of Physics-Biophysics, Georg-August University , Goettingen , Germany.
e Institute of Cellular Biochemistry, Georg-August University , Goettingen , Germany.
f Institute for Molecular Biosciences, Goethe University , Frankfurt , Germany.
g Goettingen Center for Molecular Biosciences, Georg-August-University , Goettingen , Germany.


A rapidly increasing number of RNA helicases are implicated in several distinct cellular processes, however, the modes of regulation of multifunctional RNA helicases and their recruitment to different target complexes have remained unknown. Here, we show that the distribution of the multifunctional DEAH-box RNA helicase Prp43 between its diverse cellular functions can be regulated by the interplay of its G-patch protein cofactors. We identify the orphan G-patch protein Cmg1 (YLR271W) as a novel cofactor of Prp43 and show that it stimulates the RNA binding and ATPase activity of the helicase. Interestingly, Cmg1 localizes to the cytoplasm and to the intermembrane space of mitochondria and its overexpression promotes apoptosis. Furthermore, our data reveal that different G-patch protein cofactors compete for interaction with Prp43. Changes in the expression levels of Prp43-interacting G-patch proteins modulate the cellular localization of Prp43 and G-patch protein overexpression causes accumulation of the helicase in the cytoplasm or nucleoplasm. Overexpression of several G-patch proteins also leads to defects in ribosome biogenesis that are consistent with withdrawal of the helicase from this pathway. Together, these findings suggest that the availability of cofactors and the sequestering of the helicase are means to regulate the activity of multifunctional RNA helicases and their distribution between different cellular processes.


G-patch protein; RNA helicase; protein cofactor; ribosome; splicing

[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Taylor & Francis Icon for PubMed Central
Loading ...
Support Center