Polymerase recognition of 2-thio-iso-guanine·5-methyl-4-pyrimidinone (iGs·P)--A new DD/AA base pair

Bioorg Med Chem Lett. 2016 Feb 15;26(4):1177-9. doi: 10.1016/j.bmcl.2016.01.041. Epub 2016 Jan 16.

Abstract

Polymerase specificity is reported for a previously unknown base pair with a non-standard DD/AA hydrogen bonding pattern: 2-thio-iso-guanine·5-methyl-4-pyrimidinone. Our findings suggest that atomic substitution may provide a solution for low fidelity previously associated with enzymatic copying of iso-guanine.

Keywords: Base-pair; Nucleic acids; Origins of life; Synthetic biology.

MeSH terms

  • Base Pairing
  • Base Sequence
  • Guanine / analogs & derivatives*
  • Guanine / chemistry
  • Guanine / metabolism
  • Hydrogen Bonding
  • Oligonucleotides / biosynthesis
  • Oligonucleotides / chemistry
  • Polymerase Chain Reaction
  • Pyrimidinones / chemistry
  • Pyrimidinones / metabolism*
  • Transition Temperature

Substances

  • 2-thio-iso-guanine 5-methyl-4-pyrimidinone
  • Oligonucleotides
  • Pyrimidinones
  • Guanine