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Biomed Opt Express. 2015 Dec 3;7(1):1-12. doi: 10.1364/BOE.7.000001. eCollection 2016 Jan 1.

Wavefront sensorless adaptive optics fluorescence biomicroscope for in vivo retinal imaging in mice.

Author information

1
Engineering Science, Simon Fraser University, Burnaby BC, V5A 1S6 Canada; These authors contributed equally to this work.
2
CNR-Institute for Photonics and Nanotechnology, Via Trasea 7, 35131, Padova, Italy; Hilase project, Institute of Physics AS CR v.v.i., Na Slovance 2, 18221, Prague, Czech Republic.
3
UC Davis RISE Small Animal Ocular Imaging Facility, Department of Cell Biology and Human Anatomy, University of California Davis, Davis, CA 95616 USA; Vision Science and Advanced Retinal Imaging Laboratory (VSRI), Department of Ophthalmology & Vision Science, University of California Davis, Sacramento, CA 95817 USA.
4
Engineering Science, Simon Fraser University, Burnaby BC, V5A 1S6 Canada.

Abstract

Cellular-resolution in vivo fluorescence imaging is a valuable tool for longitudinal studies of retinal function in vision research. Wavefront sensorless adaptive optics (WSAO) is a developing technology that enables high-resolution imaging of the mouse retina. In place of the conventional method of using a Shack-Hartmann wavefront sensor to measure the aberrations directly, WSAO uses an image quality metric and a search algorithm to drive the shape of the adaptive element (i.e. deformable mirror). WSAO is a robust approach to AO and it is compatible with a compact, low-cost lens-based system. In this report, we demonstrated a hill-climbing algorithm for WSAO with a variable focus lens and deformable mirror for non-invasive in vivo imaging of EGFP (enhanced green fluorescent protein) labelled ganglion cells and microglia cells in the mouse retina.

KEYWORDS:

(010.1080) Active or adaptive optics; (170.0110) Imaging systems; (170.4460) Ophthalmic optics and devices; (170.4470) Ophthalmology

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