Send to

Choose Destination
Biomed Opt Express. 2015 Dec 3;7(1):1-12. doi: 10.1364/BOE.7.000001. eCollection 2016 Jan 1.

Wavefront sensorless adaptive optics fluorescence biomicroscope for in vivo retinal imaging in mice.

Author information

Engineering Science, Simon Fraser University, Burnaby BC, V5A 1S6 Canada; These authors contributed equally to this work.
CNR-Institute for Photonics and Nanotechnology, Via Trasea 7, 35131, Padova, Italy; Hilase project, Institute of Physics AS CR v.v.i., Na Slovance 2, 18221, Prague, Czech Republic.
UC Davis RISE Small Animal Ocular Imaging Facility, Department of Cell Biology and Human Anatomy, University of California Davis, Davis, CA 95616 USA; Vision Science and Advanced Retinal Imaging Laboratory (VSRI), Department of Ophthalmology & Vision Science, University of California Davis, Sacramento, CA 95817 USA.
Engineering Science, Simon Fraser University, Burnaby BC, V5A 1S6 Canada.


Cellular-resolution in vivo fluorescence imaging is a valuable tool for longitudinal studies of retinal function in vision research. Wavefront sensorless adaptive optics (WSAO) is a developing technology that enables high-resolution imaging of the mouse retina. In place of the conventional method of using a Shack-Hartmann wavefront sensor to measure the aberrations directly, WSAO uses an image quality metric and a search algorithm to drive the shape of the adaptive element (i.e. deformable mirror). WSAO is a robust approach to AO and it is compatible with a compact, low-cost lens-based system. In this report, we demonstrated a hill-climbing algorithm for WSAO with a variable focus lens and deformable mirror for non-invasive in vivo imaging of EGFP (enhanced green fluorescent protein) labelled ganglion cells and microglia cells in the mouse retina.


(010.1080) Active or adaptive optics; (170.0110) Imaging systems; (170.4460) Ophthalmic optics and devices; (170.4470) Ophthalmology

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center