Absence of human rhinovirus and respiratory syncytial virus from bronchoalveolar lavage and bronchial biopsies of selected patients with stable chronic obstructive pulmonary disease

Styliani Giannakaki; Lida Politi; Elvira Markella Antonogiannaki; Nick Spanakis; Georgios Arsenis; Vasiliki Filaditaki; Spyridon Zakynthinos; Napoleon Karagiannidis; Athanassios Tsakris

doi:10.1186/s12931-016-0323-x

Absence of human rhinovirus and respiratory syncytial virus from bronchoalveolar lavage and bronchial biopsies of selected patients with stable chronic obstructive pulmonary disease

Respir Res. 2016 Jan 27:17:11. doi: 10.1186/s12931-016-0323-x.

Authors

Styliani Giannakaki¹, Lida Politi², Elvira Markella Antonogiannaki³, Nick Spanakis⁴, Georgios Arsenis⁵, Vasiliki Filaditaki⁶, Spyridon Zakynthinos⁷, Napoleon Karagiannidis⁸, Athanassios Tsakris⁹

Affiliations

¹ 2nd Respiratory Medicine Department, Sismanogleio-A.Fleming General Hospital, Athens, Greece. stelagiannakaki@hotmail.com.
² Department of Microbiology, Medical School, University of Athens, Athens, Greece. lpoliti@med.uoa.gr.
³ 2nd Respiratory Medicine Department, Sismanogleio-A.Fleming General Hospital, Athens, Greece. kantonogiannaki@gmail.com.
⁴ Department of Microbiology, Medical School, University of Athens, Athens, Greece. nspan@med.uoa.gr.
⁵ Department of Microbiology, Medical School, University of Athens, Athens, Greece. georgearsenis@yahoo.com.
⁶ 2nd Respiratory Medicine Department, Sismanogleio-A.Fleming General Hospital, Athens, Greece. filaditaki@yahoo.com.
⁷ 1st Department of Critical Care and Pulmonary Services, Evangelismos Hospital, Medical School, University of Athens, Athens, Greece. szakynthinos@yahoo.com.
⁸ 2nd Respiratory Medicine Department, Sismanogleio-A.Fleming General Hospital, Athens, Greece. nap@karagianidis.gr.
⁹ Department of Microbiology, Medical School, University of Athens, Athens, Greece. atsakris@med.uoa.gr.

Abstract

Previous studies have reported very different rates of human rhinovirus (HRV) and respiratory syncytial virus (RSV) genome detection in nasal and sputum samples, but not in bronchoalveolar lavage (BAL) and bronchial biopsy samples. Our study aimed to investigate the presence of HRV and RSV in the lungs of 31 consecutive patients with stable COPD (11 GOLD stage I, 11 II, and 9 III) and 22 control subjects (12 current or past smokers, and 10 non-smokers), who underwent diagnostic (e.g., lung cancer) and/or therapeutic (e.g., hemoptysis) fibreoptic bronchoscopy in a university hospital in Athens, Greece. Viral RNA of HRV and RSV were not detected in any of the samples of COPD patients or control subjects after being processed with real-time PCR.

Publication types

Editorial

MeSH terms

Aged
Bronchi / pathology
Bronchi / virology*
Bronchoalveolar Lavage Fluid / virology*
Female
Humans
Male
Middle Aged
Pulmonary Disease, Chronic Obstructive / diagnosis*
Pulmonary Disease, Chronic Obstructive / virology*
Respiratory Syncytial Viruses / isolation & purification*
Rhinovirus / isolation & purification*
Severity of Illness Index
Sputum / virology