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J Transl Med. 2016 Jan 27;14:27. doi: 10.1186/s12967-016-0776-1.

Obesity inhibits the osteogenic differentiation of human adipose-derived stem cells.

Author information

1
Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, 1430 Tulane Avenue, New Orleans, LA, 70112, USA. alin1@tulane.edu.
2
Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, 1430 Tulane Avenue, New Orleans, LA, 70112, USA. rhunter5@tulane.edu.
3
Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, 1430 Tulane Avenue, New Orleans, LA, 70112, USA. rjones25@tulane.edu.
4
Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, 1430 Tulane Avenue, New Orleans, LA, 70112, USA. abowles@tulane.edu.
5
Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, 1430 Tulane Avenue, New Orleans, LA, 70112, USA. mdutreil@tulane.edu.
6
Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, 1430 Tulane Avenue, New Orleans, LA, 70112, USA. dgaupp@tulane.edu.
7
Department of Biological and Agricultural Engineering, Louisiana State University, Baton Rouge, LA, 70803, USA. danielhayes@lsu.edu.
8
Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, 1430 Tulane Avenue, New Orleans, LA, 70112, USA. jgimble@tulane.edu.
9
LaCell LLC, New Orleans, LA, 70112, USA. jgimble@tulane.edu.
10
Department of Surgery, Tulane University School of Medicine, New Orleans, LA, 70112, USA. jgimble@tulane.edu.
11
Department of Surgery, Division of Plastic Surgery, University of Michigan, Ann Arbor, MI, USA. blevi@med.umich.edu.
12
Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, 70803, USA. mcnulty@lsu.edu.
13
Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, 1430 Tulane Avenue, New Orleans, LA, 70112, USA. bbunnell@tulane.edu.
14
Department of Pharmacology, Tulane University School of Medicine, New Orleans, LA, 70112, USA. bbunnell@tulane.edu.

Abstract

BACKGROUND:

Craniomaxillofacial defects secondary to trauma, tumor resection, or congenital malformations are frequent unmet challenges, due to suboptimal alloplastic options and limited autologous tissues such as bone. Significant advances have been made in the application of adipose-derived stem/stromal cells (ASCs) in the pre-clinical and clinical settings as a cell source for tissue engineering approaches. To fully realize the translational potential of ASCs, the identification of optimal donors for ASCs will ensure the successful implementation of these cells for tissue engineering approaches. In the current study, the impact of obesity on the osteogenic differentiation of ASCs was investigated.

METHODS:

ASCs isolated from lean donors (body mass index <25; lnASCs) and obese donors (body mass index >30; obASCs) were induced with osteogenic differentiation medium as monolayers in an estrogen-depleted culture system and on three-dimensional scaffolds. Critical size calvarial defects were generated in male nude mice and treated with scaffolds implanted with lnASCs or obASCs.

RESULTS:

lnASCs demonstrated enhanced osteogenic differentiation in monolayer culture system, on three-dimensional scaffolds, and for the treatment of calvarial defects, whereas obASCs were unable to induce similar levels of osteogenic differentiation in vitro and in vivo. Gene expression analysis of lnASCs and obASCs during osteogenic differentiation demonstrated higher levels of osteogenic genes in lnASCs compared to obASCs.

CONCLUSION:

Collectively, these results indicate that obesity reduces the osteogenic differentiation capacity of ASCs such that they may have a limited suitability as a cell source for tissue engineering.

PMID:
26818763
PMCID:
PMC4730660
DOI:
10.1186/s12967-016-0776-1
[Indexed for MEDLINE]
Free PMC Article

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