Incorporation of an aggregation-induced-emissive tetraphenylethene derivative into cationic gene delivery vehicles manifested the nuclear translocation of uncomplexed DNA

Chem Commun (Camb). 2016 Mar 11;52(20):3907-10. doi: 10.1039/c5cc09836j. Epub 2016 Jan 27.

Abstract

A fluorophore displaying aggregation-induced emission was introduced at the terminus of branched polyethylenimine (PEI). The formulated polyplex not only demonstrated an improved safety profile and preserved transfection activity but also importantly indicated that the uncomplexed naked DNA rather than the polyplexes translocated into the nucleus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbocyanines / chemistry*
  • Cations / chemistry
  • DNA / chemistry*
  • Ethylenes / chemistry*
  • Fluorescent Dyes / chemistry*
  • Gene Transfer Techniques*
  • Molecular Structure
  • Particle Size
  • Plasmids
  • Surface Properties

Substances

  • Carbocyanines
  • Cations
  • Ethylenes
  • Fluorescent Dyes
  • cyanine dye 5
  • DNA