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Funct Integr Genomics. 2016 Mar;16(2):115-26. doi: 10.1007/s10142-015-0471-y. Epub 2016 Jan 27.

The wheat homolog of putative nucleotide-binding site-leucine-rich repeat resistance gene TaRGA contributes to resistance against powdery mildew.

Author information

1
College of Life Science, Shanxi Agricultural University, Taigu, Shanxi, 030801, China.
2
National Ministry of Education Key Laboratory of Integrated Management of Crop Diseases and Insect Pests, Nanjing Agricultural University, Nanjing, 210095, China.
3
National Ministry of Education Key Laboratory of Integrated Management of Crop Diseases and Insect Pests, Nanjing Agricultural University, Nanjing, 210095, China. hsdong@njau.edu.cn.

Abstract

Powdery mildew, one of the most destructive wheat diseases worldwide, is caused by Blumeria graminis f. sp. tritici (Bgt), a fungal species with a consistently high mutation rate that makes individual resistance (R) genes ineffective. Therefore, effective resistance-related gene cloning is vital for breeding and studying the resistance mechanisms of the disease. In this study, a putative nucleotide-binding site-leucine-rich repeat (NBS-LRR) R gene (TaRGA) was cloned using a homology-based cloning strategy and analyzed for its effect on powdery mildew disease and wheat defense responses. Real-time reverse transcription-PCR (RT-PCR) analyses revealed that a Bgt isolate 15 and salicylic acid stimulation significantly induced TaRGA in the resistant variety. Furthermore, the silencing of TaRGA in powdery mildew-resistant plants increased susceptibility to Bgt15 and prompted conidia propagation at the infection site. However, the expression of TaRGA in leaf segments after single-cell transient expression assay highly increased the defense responses to Bgt15 by enhancing callose deposition and phenolic autofluorogen accumulation at the pathogen invading sites. Meanwhile, the expression of pathogenesis-related genes decreased in the TaRGA-silenced plants and increased in the TaRGA-transient-overexpressing leaf segments. These results implied that the TaRGA gene positively regulates the defense response to powdery mildew disease in wheat.

KEYWORDS:

Functional identification; NBS-LRR; Resistance gene; Wheat

PMID:
26815536
DOI:
10.1007/s10142-015-0471-y
[Indexed for MEDLINE]

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