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J Pharmacol Sci. 2016 Jan;130(1):15-23. doi: 10.1016/j.jphs.2015.12.002. Epub 2015 Dec 15.

Ameliorative effects of tannic acid on carbon tetrachloride-induced liver fibrosis in vivo and in vitro.

Author information

1
Department of Pharmacy, The Fourth Hospital of Hebei Medical University, No. 12, Jiankang Road, Shijiazhuang, 050011, Hebei, China. Electronic address: chux2014@126.com.
2
Hebei Medical University, No. 361, East Zhongshan Road, Shijiazhuang, 050017, Hebei, China.
3
Department of Respiration, The Third Hospital of Shijiazhuang, No. 15, South Sports Street, Shijiazhuang, 050011, Hebei, China.
4
Hebei University of Chinese Medicine, No. 3, Xingyuan Road, Shijiazhuang, 050200, Hebei, China; Hebei Key Laboratory of Integrative Medicine on Liver-Kidney Patterns, China.
5
Key Laboratory of Drug Metabolism & Pharmacokinetics, China Pharmaceutical University, No. 1, Shennong Road of the Central Door, Nanjing, 210038, Jiangsu, China.
6
Hebei University of Chinese Medicine, No. 3, Xingyuan Road, Shijiazhuang, 050200, Hebei, China.
7
Chest Hospital of Hebei, No. 372, North Shengli Street, Shijiazhuang, 050041, Hebei, China.
8
Intensive Care Unit, Air Force General Hospital, No. 30, Fucheng Road, Haidian, 100142, Beijing, China.

Abstract

We investigated the ameliorative effects and potential mechanisms of tannic acid (TA) in carbon tetrachloride (CCl4)-intoxicated mice and hepatic stellate cells (HSCs). Liver fibrosis was observed in CCl4 (800 ml/kg)-induced mice, and high viability was observed in CCl4 (10 mM)-intoxicated HSCs. Pre-treatment of mice with TA (25 or 50 g/kg/day) significantly ameliorated hepatic morphology and coefficient values and reduced the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), the concentrations of malondialdehyde (MDA) and serum levels of endothelin-1 (ET-1). In addition, TA increased the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and endothelial nitric oxide synthase (eNOS) and the serum level of NO. Moreover, TA reduced the expression of angiotensin II receptor-1 (ATR-1), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), caspase-3, c-fos, c-jun, the ratio of Bax/bcl-2, tissue inhibitor of metalloproteinase-1 (TIMP-1) and TA increased matrix metal proteinase-9 (MMP-9), matrix metalloproteinase-1 (MMP-1). Furthermore, TA (0.01 μM, 0.1 μM or 1 μM) decreased the TIMP-1/MMP-1 ratio and reduced the viability of HSCs. These results indicated that TA exerts significant liver-protective effects in mice with CCl4-induced liver fibrosis. The potential mechanism may rely on the inhibition of collagen accumulation, oxidative stress, inflammation and apoptosis.

KEYWORDS:

Anti-Apoptosis; Anti-Inflammation; Anti-fibrosis; Anti-oxidation; Tannic acid

PMID:
26810570
DOI:
10.1016/j.jphs.2015.12.002
[Indexed for MEDLINE]
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