EV71 virus-like particles produced by co-expression of capsid proteins in yeast cells elicit humoral protective response against EV71 lethal challenge

BMC Res Notes. 2016 Jan 25:9:42. doi: 10.1186/s13104-015-1780-x.

Abstract

Background: Enterovirus 71 (EV71) is the most common causative pathogens of hand, foot and mouth disease (HFMD) associated with severe neurological complications. There is a great need to develop prophylactic vaccine against EV71 infection.

Results: EV71 virus-like particle (VLP) was produced in yeast expression system by the co-expression of four EV71 structural proteins VP1-VP4. Immunization with the recombinant VLPs elicited potent anti-EV71 antibody responses in adult mice and anti-VLP sera were able to neutralize EV71 virus in vitro. Neonatal mice model demonstrated VLP immunization conferred protection to suckling mice against the lethal viral challenge.

Conclusions: Co-expression of four EV71 structural proteins VP1-VP4 in yeast expression systems is an effective method to produce EV71 VLPs. VLP-based vaccine shows great potential to prevent EV71 infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Antibodies, Neutralizing / biosynthesis*
  • Antibodies, Viral / biosynthesis*
  • Capsid Proteins / administration & dosage
  • Capsid Proteins / genetics
  • Capsid Proteins / immunology
  • Disease Models, Animal
  • Enterovirus A, Human / drug effects*
  • Enterovirus A, Human / growth & development
  • Enterovirus A, Human / immunology
  • Female
  • Gene Expression
  • Hand, Foot and Mouth Disease / immunology
  • Hand, Foot and Mouth Disease / prevention & control*
  • Hand, Foot and Mouth Disease / virology
  • Immunity, Humoral / drug effects
  • Mice
  • Mice, Inbred BALB C
  • Neutralization Tests
  • Plasmids / chemistry
  • Plasmids / immunology
  • Protein Isoforms / administration & dosage
  • Protein Isoforms / genetics
  • Protein Isoforms / immunology
  • Recombinant Proteins / administration & dosage
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Vaccination
  • Vaccines, Virus-Like Particle / administration & dosage
  • Vaccines, Virus-Like Particle / genetics
  • Vaccines, Virus-Like Particle / immunology*
  • Viral Vaccines / administration & dosage
  • Viral Vaccines / genetics
  • Viral Vaccines / immunology*

Substances

  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Capsid Proteins
  • Protein Isoforms
  • Recombinant Proteins
  • VP1 protein, Foot-and-mouth disease virus
  • VP2 protein, Foot-and-mouth disease virus
  • VP3 protein, Foot-and-mouth disease virus
  • Vaccines, Virus-Like Particle
  • Viral Vaccines