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ACS Nano. 2016 Feb 23;10(2):1802-9. doi: 10.1021/acsnano.5b07584. Epub 2016 Jan 28.

Integrated Magneto-Electrochemical Sensor for Exosome Analysis.

Author information

1
Center for Systems Biology, Massachusetts General Hospital , 185 Cambridge Street, CPZN 5206, Boston, Massachusetts 02114, United States.
2
Massachusetts General Hospital Cancer Center , Boston, Massachusetts 02114, United States.
3
Department of Systems Biology, Harvard Medical School , 200 Longwood Avenue, Boston, Massachusetts 02115, United States.

Abstract

Extracellular vesicles, including exosomes, are nanoscale membrane particles that carry molecular information on parental cells. They are being pursued as biomarkers of cancers that are difficult to detect or serially follow. Here we present a compact sensor technology for rapid, on-site exosome screening. The sensor is based on an integrated magneto-electrochemical assay: exosomes are immunomagnetically captured from patient samples and profiled through electrochemical reaction. By combining magnetic enrichment and enzymatic amplification, the approach enables (i) highly sensitive, cell-specific exosome detection and (ii) sensor miniaturization and scale-up for high-throughput measurements. As a proof-of-concept, we implemented a portable, eight-channel device and applied it to screen extracellular vesicles in plasma samples from ovarian cancer patients. The sensor allowed for the simultaneous profiling of multiple protein markers within an hour, outperforming conventional methods in assay sensitivity and speed.

KEYWORDS:

cancer; diagnostics; electrochemical sensing; extracellular vesicles; point-of-care

PMID:
26808216
PMCID:
PMC4802494
DOI:
10.1021/acsnano.5b07584
[Indexed for MEDLINE]
Free PMC Article

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