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Am J Transl Res. 2015 Nov 15;7(11):2212-22. eCollection 2015.

miR-485-5p acts as a negative regulator in gastric cancer progression by targeting flotillin-1.

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Department of Digestive Diseases, Affiliated Hospital of Luzhou Medical College Luzhou, Sichuan, China.
Drug and Functional Food Center, Luzhou Medical College Luzhou, Sichuan, China.
Department of Digestive Diseases, The Second Affiliated Hospital of Haerbin Medical University Haerbin, China.


MicroRNAs (miRNAs) play important roles in cancer progression including gastric cancer. miR-485-5p is reported as a potential suppressor in breast cancer, but its expression, cellular function and clinic features in gastric cancer is not known. In our study, we found that miR-485-5p expression was down-regulated in gastric cancer cell lines. miR-485-5p could inhibit gastric cancer cell growth in vitro and in vivo. We also found that miR-485-5p suppressed gastric cancer cell metastasis and sphere formation. It was confirmed flotillin-1 (Flot1) as a direct target of miR-485-5p, and up-regulation of miR-485-5p could decrease expression of Flot1 in gastric cancer cells. Further investigation showed that ectopic expression of Flot1 partially reversed the inhibition effect of enforced miR-485-5p expression on the malignant phenotypes of gastric cancer cells. The low expression of miR-485-5p in gastric cancer tissues was related to advanced clinical features and poorer prognosis. Our study suggested that miR-485-5p could be a potential prognostic marker and functions as a tumor suppressor in human gastric cancer by post-transcriptionally targeting Flot1.


Flot1; Gastric cancer; miR-485-5p


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