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Int J Biol Macromol. 2016 May;86:224-32. doi: 10.1016/j.ijbiomac.2016.01.002. Epub 2016 Jan 21.

Extraction, characterization and antioxidant activities of polysaccharides of Chuanminshen violaceum.

Author information

1
Department of Agricultural Products Processing and Storage Engineering, College of Food Science, Sichuan Agricultural University, Ya'an 625014, China. Electronic address: donghmin@outlook.com.
2
Department of Agricultural Products Processing and Storage Engineering, College of Food Science, Sichuan Agricultural University, Ya'an 625014, China. Electronic address: zhangqing@sicau.edu.cn.
3
Department of Agricultural Products Processing and Storage Engineering, College of Food Science, Sichuan Agricultural University, Ya'an 625014, China. Electronic address: 15283522791@163.com.
4
Department of Agricultural Products Processing and Storage Engineering, College of Food Science, Sichuan Agricultural University, Ya'an 625014, China. Electronic address: 765416741@qq.com.
5
Department of Agricultural Products Processing and Storage Engineering, College of Food Science, Sichuan Agricultural University, Ya'an 625014, China. Electronic address: lwj18728158690@163.com.
6
Department of Agricultural Products Processing and Storage Engineering, College of Food Science, Sichuan Agricultural University, Ya'an 625014, China. Electronic address: hjingliu@163.com.
7
Department of Agricultural Products Processing and Storage Engineering, College of Food Science, Sichuan Agricultural University, Ya'an 625014, China. Electronic address: Lee909293@163.com.
8
School of Printing and Packaging, Wuhan University, Wuhan 430072, China. Electronic address: xiongyabo1030@163.com.
9
Department of Agricultural Products Processing and Storage Engineering, College of Food Science, Sichuan Agricultural University, Ya'an 625014, China. Electronic address: qinwen@sicau.edu.cn.

Abstract

A single factor experiment and Box-Behnken design were applied to optimize the microwave-assisted extraction (MAE) of Chuanminshen violaceum polysaccharides (CVPs). The CVPs yield was 34.59 ± 0.51%, which was in accordance with the predicted value of 35.54%, under the following optimum conditions: microwave power of 466 W, extraction temperature of 64.5 °C, extraction time of 15 min, and water-raw material ratio of 40 mL/g. Two CVPs fractions (CVPs-I and CVPs-II) were obtained via stepwise ethanol precipitation. CVPs-II was further purified using DEAE cellulose-52 chromatography to obtain the major fraction of CVPs-IIa. Fourier transform infrared spectroscopy (FTIR) and GC-MS analysis revealed that crude CVPs, CVPs-I, and CVPs-II were neutral polysaccharides and were mainly composed of glucose and galactose. The molecular weights of CVPs-I and CVPs-IIa were 233.69 and 11.02 kDa, respectively. Furthermore, all samples exhibited certain antioxidant activity or reducing power to scavenge the DPPH and ABTS radicals, among which CVPs-II was the strongest. In conclusion, MAE is an efficient method for extracting CVPs of C. violaceum, and this plant root has the potential to be explored as a source of natural antioxidants.

KEYWORDS:

Chuanminshen violaceum; Microwave-assisted extraction; Polysaccharide

PMID:
26806648
DOI:
10.1016/j.ijbiomac.2016.01.002
[Indexed for MEDLINE]

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