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Biochem Biophys Res Commun. 2016 Feb 19;470(4):838-44. doi: 10.1016/j.bbrc.2016.01.122. Epub 2016 Jan 22.

miR-625 suppresses cell proliferation and migration by targeting HMGA1 in breast cancer.

Author information

1
Department of Breast Surgery, Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong Province, China.
2
Department of Breast Surgery, Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong Province, China. Electronic address: 1072241978@qq.com.
3
Department of Hepatobiliary and Pancreas Surgery, Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong Province, China. Electronic address: leoliping@aliyun.com.

Abstract

Dysregulation of microRNA contributes to the high incidence and mortality of breast cancer. Here, we show that miR-625 was frequently down-regulated in breast cancer. Decrease of miR-625 was closely associated with estrogen receptor (P = 0.004), human epidermal growth factor receptor 2 (P = 0.003) and clinical stage (P = 0.001). Kaplan-Meier and multivariate analyses indicated miR-625 as an independent factor for unfavorable prognosis (hazard ratio = 2.654, 95% confident interval: 1.300-5.382, P = 0.007). Re-expression of miR-625 impeded, whereas knockdown of miR-625 enhanced cell viabilities and migration abilities in breast cancer cells. HMGA1 was confirmed as a direct target of miR-625. The expressions of HMGA1 mRNA and protein were induced by miR-625 mimics, but reduced by miR-625 inhibitor. Re-introduction of HMGA1 in cells expressing miR-625 distinctly abrogated miR-625-mediated inhibition of cell growth. Taken together, our data demonstrate that miR-625 suppresses cell proliferation and migration by targeting HMGA1 and suggest miR-625 as a promising prognostic biomarker and a potential therapeutic target for breast cancer.

KEYWORDS:

Breast cancer; HMGA1; Migration; Proliferation; miR-625

PMID:
26806308
DOI:
10.1016/j.bbrc.2016.01.122
[Indexed for MEDLINE]

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