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Mol Cell. 2016 Feb 4;61(3):352-363. doi: 10.1016/j.molcel.2015.12.023. Epub 2016 Jan 21.

A 3' UTR-Derived Small RNA Provides the Regulatory Noncoding Arm of the Inner Membrane Stress Response.

Author information

1
RNA Biology Group, Institute for Molecular Infection Biology (IMIB), University of Würzburg, Josef-Schneider-Strasse 2, 97080 Würzburg, Germany.
2
RNA Biology Group, Institute for Molecular Infection Biology (IMIB), University of Würzburg, Josef-Schneider-Strasse 2, 97080 Würzburg, Germany. Electronic address: joerg.vogel@uni-wuerzburg.de.

Abstract

Small RNAs (sRNAs) from conserved noncoding genes are crucial regulators in bacterial signaling pathways but have remained elusive in the Cpx response to inner membrane stress. Here we report that an alternative biogenesis pathway releasing the conserved mRNA 3' UTR of stress chaperone CpxP as an ∼60-nt sRNA provides the noncoding arm of the Cpx response. This so-called CpxQ sRNA, generated by general mRNA decay through RNase E, acts as an Hfq-dependent repressor of multiple mRNAs encoding extracytoplasmic proteins. Both CpxQ and the Cpx pathway are required for cell survival under conditions of dissipation of membrane potential. Our discovery of CpxQ illustrates how the conversion of a transcribed 3' UTR into an sRNA doubles the output of a single mRNA to produce two factors with spatially segregated functions during inner membrane stress: a chaperone that targets problematic proteins in the periplasm and a regulatory RNA that dampens their synthesis in the cytosol.

KEYWORDS:

3′ UTR; Cpx pathway; CpxP; CpxQ; Hfq; NhaB; RNase E; envelope stress; membrane potential; noncoding RNA

PMID:
26805574
DOI:
10.1016/j.molcel.2015.12.023
[Indexed for MEDLINE]
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