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Regul Toxicol Pharmacol. 2016 Apr;76:57-62. doi: 10.1016/j.yrtph.2016.01.011. Epub 2016 Jan 21.

Antimicrobial activities of ethanol and butanol fractions of white rose petal extract.

Author information

1
College of Veterinary Medicine, Chungbuk National University, Cheongju, South Korea.
2
Department of Biomedical Laboratory Sciences, Semyung University, Jecheon, South Korea.
3
Department of Food Science and Technology, Chungbuk National University, Cheongju, South Korea.
4
Department of Marine Molecular Biotechnology, Gangneung-Wonju National University, Gangneung, South Korea.
5
Central Research Institute, Dr. Chung's Food Co. Ltd., Cheongju, South Korea.
6
Department of Biomedical Laboratory Sciences, Yonsei University, Wonju, South Korea.
7
College of Veterinary Medicine, Chungbuk National University, Cheongju, South Korea. Electronic address: solar93@cbu.ac.kr.

Abstract

White rose (Rosa hybrida) petals were extracted with ethanol (EtOH) or butanol (BuOH), and tested for their antimicrobial activities against two species of Gram-positive bacteria, six species of Gram-negative bacteria, and two species of fungi. On in vitro antimicrobial assays, Helicobacter pylori and Propionibacterium acnes were highly susceptible to white rose petal extract (WRPE)-EtOH and WRPE-BuOH, leading to minimal inhibitory concentrations of 100 and 10 μg/mL for H. pylori and 400 and 40 μg/mL for P. acnes, respectively. In in vivo experiments, C57BL/6 mice were infected with H. pylori by intragastric inoculation (1 × 10(8) CFU/mouse) 3 times, and orally treated twice a day for 14 days with WRPE-EtOH and WRPE-BuOH. On a CLO kit assay, 200 mg/kg of WRPE-EtOH fully eliminated the bacteria from the gastric mucosa, and the effect of 100 mg/kg of ethanol fraction was similar to pantoprazole (30 mg/kg), displaying 75% elimination. WRPE-BuOH was more effective, exhibiting 75% elimination at 20 mg/kg. The CLO test results were confirmed by bacterial identification. WRPE-EtOH and WRPE-BuOH inhibited the growth of various bacteria and fungi, and in particular, they effectively killed H. pylori and eliminated the bacteria from the mouse stomach. The results indicate that WRPE-EtOH and WRPE-BuOH could be good candidates for the elimination of H. pylori.

KEYWORDS:

Antimicrobial activity; Butanol fraction; Ethanol fraction; Helicobacter pylori; Propionibacterium acnes; White rose petal extract

PMID:
26802535
DOI:
10.1016/j.yrtph.2016.01.011
[Indexed for MEDLINE]

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