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Cell. 1989 Nov 3;59(3):461-75.

Villin induces microvilli growth and actin redistribution in transfected fibroblasts.

Author information

1
Institut Pasteur, D├ępartement de Biologie Mol├ęculaire, Paris, France.

Abstract

The function of villin, an actin-binding protein, has been investigated by transfecting fibroblasts with cloned human cDNAs encoding wild-type villin or functional villin domains. Synthesis of large amounts of villin induced the growth of numerous long microvilli on cell surfaces together with the redistribution of F-actin. These microvilli contained a cytoskeleton of F-actin, and their appearance was frequently accompanied by the disappearance of stress fibers. The complete villin gene sequence was required to exert its morphogenic effect. Villin lacking one actin-binding domain (113 amino acids), located at its carboxyterminal end, did not induce growth if microvilli or stress fiber disruption. Our results indicate that villin plays a key role in vivo in the morphogenesis of microvilli.

PMID:
2680107
DOI:
10.1016/0092-8674(89)90030-5
[Indexed for MEDLINE]

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