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J Exp Bot. 2016 Mar;67(6):1759-68. doi: 10.1093/jxb/erv566. Epub 2016 Jan 21.

Duplication of OsHAP family genes and their association with heading date in rice.

Author information

1
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.
2
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China Hubei Collaborative Innovation Center for Grain Industry , China yzxing@mail.hzau.edu.cn.

Abstract

Heterotrimeric Heme Activator Protein (HAP) family genes are involved in the regulation of flowering in plants. It is not clear how many HAP genes regulate heading date in rice. In this study, we identified 35 HAP genes, including seven newly identified genes, and performed gene duplication and candidate gene-based association analyses. Analyses showed that segmental duplication and tandem duplication are the main mechanisms of HAP gene duplication. Expression profiling and functional identification indicated that duplication probably diversifies the functions of HAP genes. A nucleotide diversity analysis revealed that 13 HAP genes underwent selection. A candidate gene-based association analysis detected four HAP genes related to heading date. An investigation of transgenic plants or mutants of 23 HAP genes confirmed that overexpression of at least four genes delayed heading date under long-day conditions, including the previously cloned Ghd8/OsHAP3H. Our results indicate that the large number of HAP genes in rice was mainly produced by gene duplication, and a few HAP genes function to regulate heading date. Selection of HAP genes is probably caused by their diverse functions rather than regulation of heading.

KEYWORDS:

Association analysis; OsHAP.; expression profiling; gene duplication; heading date; nucleotide diversity

PMID:
26798026
PMCID:
PMC4783360
DOI:
10.1093/jxb/erv566
[Indexed for MEDLINE]
Free PMC Article

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