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Anim Reprod Sci. 2016 Mar;166:122-7. doi: 10.1016/j.anireprosci.2016.01.011. Epub 2016 Jan 11.

Quantification of leptin in seminal plasma of buffalo bulls and its correlation with antioxidant status, conventional and computer-assisted sperm analysis (CASA) semen variables.

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ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana, India. Electronic address:
ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana, India.
ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana, India; Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India.


The present study is the first to quantify leptin in seminal plasma of buffalo and investigate its relationship with seminal attributes. Ten ejaculates each from 10 Murrah buffalo bulls were collected. Semen quality variables such as semen volume, sperm concentration, sperm abnormalities, membrane integrity, antioxidant enzyme activities (superoxide dismutase, catalase and total antioxidant capacity), malondialdehyde (MDA) concentration, as well as sperm kinetics and motility variables were evaluated. The leptin concentration in serum and seminal plasma were estimated by the ELISA method. Bulls were classified in two groups on the basis of sperm concentration with Group I having >800 million sperm/mL and Group II <500 million sperm/mL. Greater (P<0.05) mean sperm abnormalities, seminal leptin concentrations and MDA concentrations were recorded in Group II than Group I. The seminal leptin was positively correlated with sperm abnormalities and MDA concentration while being negatively correlated with sperm concentration, but there was no correlation with sperm kinetic and motility variables, sperm membrane integrity and seminal plasma antioxidant enzyme activity. Thus, the data suggest that seminal leptin has a role in spermatogenesis and can be used as a marker for spermatogenesis to predict the capacity of buffalo bulls for semen production.


Buffalo; CASA; Cryopreservation; Leptin; Semen quality

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