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Proc Natl Acad Sci U S A. 2016 Feb 2;113(5):1447-52. doi: 10.1073/pnas.1525122113. Epub 2016 Jan 20.

PP2A-3 interacts with ACR4 and regulates formative cell division in the Arabidopsis root.

Author information

1
Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium;
2
Roy J. Carver Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011;
3
Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, United Kingdom;
4
Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium; Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium; Department of Biochemistry, Ghent University, B-9000 Ghent, Belgium;
5
Multidisciplinary Centre for Integrative Biology, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, United Kingdom;
6
Department of Medicine, Washington University School of Medicine, St. Louis, MO 63130;
7
The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, United Kingdom;
8
Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium; ive.desmet@psb.vib-ugent.be marc.vanmontagu@ugent.be.
9
Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium; Department of Biochemistry, Ghent University, B-9000 Ghent, Belgium;
10
Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium; Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, United Kingdom; Centre for Plant Integrative Biology, University of Nottingham, Loughborough LE12 5RD, United Kingdom ive.desmet@psb.vib-ugent.be marc.vanmontagu@ugent.be.

Abstract

In plants, the generation of new cell types and tissues depends on coordinated and oriented formative cell divisions. The plasma membrane-localized receptor kinase ARABIDOPSIS CRINKLY 4 (ACR4) is part of a mechanism controlling formative cell divisions in the Arabidopsis root. Despite its important role in plant development, very little is known about the molecular mechanism with which ACR4 is affiliated and its network of interactions. Here, we used various complementary proteomic approaches to identify ACR4-interacting protein candidates that are likely regulators of formative cell divisions and that could pave the way to unraveling the molecular basis behind ACR4-mediated signaling. We identified PROTEIN PHOSPHATASE 2A-3 (PP2A-3), a catalytic subunit of PP2A holoenzymes, as a previously unidentified regulator of formative cell divisions and as one of the first described substrates of ACR4. Our in vitro data argue for the existence of a tight posttranslational regulation in the associated biochemical network through reciprocal regulation between ACR4 and PP2A-3 at the phosphorylation level.

KEYWORDS:

columella; kinase; phosphatase; phosphorylation; stem cells

PMID:
26792519
PMCID:
PMC4747734
DOI:
10.1073/pnas.1525122113
[Indexed for MEDLINE]
Free PMC Article

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