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J Transl Med. 2016 Jan 20;14:19. doi: 10.1186/s12967-016-0771-6.

Mitochondrial DNA variants correlate with symptoms in myalgic encephalomyelitis/chronic fatigue syndrome.

Author information

1
Division of Nutritional Sciences, Cornell University, Ithaca, NY, 14853, USA. pdb87@cornell.edu.
2
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, 14853, USA. ag297@cornell.edu.
3
Department of Biological Statistics and Computational Biology, Cornell University, Ithaca, NY, 14853, USA. ky297@cornell.edu.
4
Department of Biological Statistics and Computational Biology, Cornell University, Ithaca, NY, 14853, USA. ak735@cornell.edu.
5
Division of Nutritional Sciences, Cornell University, Ithaca, NY, 14853, USA. zg27@cornell.edu.
6
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, 14853, USA. mrh5@cornell.edu.

Abstract

BACKGROUND:

Mitochondrial dysfunction has been hypothesized to occur in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS), a disease characterized by fatigue, cognitive difficulties, pain, malaise, and exercise intolerance. We investigated whether haplogroup, single nucleotide polymorphisms (SNPs), or heteroplasmy of mitochondrial DNA (mtDNA) were associated with health status and/or symptoms.

METHODS:

Illumina sequencing of PCR-amplified mtDNA was performed to analyze sequence and extent of heteroplasmy of mtDNAs of 193 cases and 196 age- and gender-matched controls from DNA samples collected by the Chronic Fatigue Initiative. Association testing was carried out to examine possible correlations of mitochondrial sequences with case/control status and symptom constellation and severity as reported by subjects on Short Form-36 and DePaul Symptom Questionnaires.

RESULTS:

No ME/CFS subject exhibited known disease-causing mtDNA mutations. Extent of heteroplasmy was low in all subjects. Although no association between mtDNA SNPs and ME/CFS vs. healthy status was observed, haplogroups J, U and H as well as eight SNPs in ME/CFS cases were significantly associated with individual symptoms, symptom clusters, or symptom severity.

CONCLUSIONS:

Analysis of mitochondrial genomes in ME/CFS cases indicates that individuals of a certain haplogroup or carrying specific SNPs are more likely to exhibit certain neurological, inflammatory, and/or gastrointestinal symptoms. No increase in susceptibility to ME/CFS of individuals carrying particular mitochondrial genomes or SNPs was observed.

PMID:
26791940
PMCID:
PMC4719218
DOI:
10.1186/s12967-016-0771-6
[Indexed for MEDLINE]
Free PMC Article

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