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Sensors (Basel). 2016 Jan 20;16(1). pii: E96. doi: 10.3390/s16010096.

Use of Piezoelectric Immunosensors for Detection of Interferon-Gamma Interaction with Specific Antibodies in the Presence of Released-Active Forms of Antibodies to Interferon-Gamma.

Author information

1
OOO "NPF "MATERIA MEDICA HOLDING", 3rd Samotyochny per., 9, 127473 Moscow, Russian. GavrilovaES@materiamedica.ru.
2
Lipetsk State Technical University, Moskovskaya ul, 30, 398006 Lipetsk, Russian. voronezkaya@gmail.com.
3
OOO "NPF "MATERIA MEDICA HOLDING", 3rd Samotyochny per., 9, 127473 Moscow, Russian. PokhilSE@materiamedica.ru.
4
OOO "NPF "MATERIA MEDICA HOLDING", 3rd Samotyochny per., 9, 127473 Moscow, Russian. LubshinaDV@materiamedica.ru.
5
OOO "NPF "MATERIA MEDICA HOLDING", 3rd Samotyochny per., 9, 127473 Moscow, Russian. MashkinaMV@materiamedica.ru.
6
OOO "NPF "MATERIA MEDICA HOLDING", 3rd Samotyochny per., 9, 127473 Moscow, Russian. ShulgaDP@materiamedica.ru.
7
OOO "NPF "MATERIA MEDICA HOLDING", 3rd Samotyochny per., 9, 127473 Moscow, Russian. BorshchevaAA@materiamedica.ru.
8
OOO "NPF "MATERIA MEDICA HOLDING", 3rd Samotyochny per., 9, 127473 Moscow, Russian. TarasovSA@materiamedica.ru.
9
Lipetsk State Technical University, Moskovskaya ul, 30, 398006 Lipetsk, Russian. ermolaeva@stu.lipetsk.ru.
10
OOO "NPF "MATERIA MEDICA HOLDING", 3rd Samotyochny per., 9, 127473 Moscow, Russian. Nauka@materiamedica.ru.

Abstract

In preliminary ELISA studies where released-active forms (RAF) of antibodies (Abs) to interferon-gamma (IFNg) were added to the antigen-antibody system, a statistically significant difference in absorbance signals obtained in their presence in comparison to placebo was observed. A piezoelectric immunosensor assay was developed to support these data and investigate the effects of RAF Abs to IFNg on the specific interaction between Abs to IFNg and IFNg. The experimental conditions were designed and optimal electrode coating, detection circumstances and suitable chaotropic agents for electrode regeneration were selected. The developed technique was found to provide high repeatability, intermediate precision and specificity. The difference between the analytical signals of RAF Ab samples and those of the placebo was up to 50.8%, whereas the difference between non-specific controls and the placebo was within 5%-6%. Thus, the piezoelectric immunosensor as well as ELISA has the potential to be used for detecting the effects of RAF Abs to IFNg on the antigen-antibody interaction, which might be the result of RAF's ability to modify the affinity of IFNg to specific/related Abs.

KEYWORDS:

interferon-gamma; piezoelectric immunosensors; released-active forms of antibodies

PMID:
26791304
PMCID:
PMC4732129
DOI:
10.3390/s16010096
[Indexed for MEDLINE]
Free PMC Article

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