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Oxid Med Cell Longev. 2016;2016:1689602. doi: 10.1155/2016/1689602. Epub 2015 Dec 14.

Berberine Attenuates Myocardial Ischemia/Reperfusion Injury by Reducing Oxidative Stress and Inflammation Response: Role of Silent Information Regulator 1.

Author information

  • 1Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
  • 2Beijing Aortic Disease Center, Anzhen Hospital, Capital Medical University, Beijing 100029, China.
  • 3Department of Biomedical Engineering, Fourth Military Medical University, Xi'an 710032, China.
  • 4School of Clinical Medicine, Ningxia Medical University, Yinchuan 750004, China.
  • 5College of Life Science, Northwest University, Xi'an 710069, China.
  • 6Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.

Abstract

Berberine (BBR) exerts potential protective effect against myocardial ischemia/reperfusion (MI/R) injury. Activation of silent information regulator 1 (SIRT1) signaling attenuates MI/R injury by reducing oxidative damage and inflammation response. This study investigated the antioxidative and anti-inflammatory effects of BBR treatment in MI/R condition and elucidated its potential mechanisms. Sprague-Dawley rats were treated with BBR in the absence or presence of the SIRT1 inhibitor sirtinol (Stnl) and then subjected to MI/R injury. BBR conferred cardioprotective effects by improving postischemic cardiac function, decreasing infarct size, reducing apoptotic index, diminishing serum creatine kinase and lactate dehydrogenase levels, upregulating SIRT1, Bcl-2 expressions, and downregulating Bax and caspase-3 expressions. Stnl attenuated these effects by inhibiting SIRT1 signaling. BBR treatment also reduced myocardium superoxide generation, gp91(phox) expression, malondialdehyde (MDA) level, and cardiac inflammatory markers and increased myocardium superoxide dismutase (SOD) level. However, these effects were also inhibited by Stnl. Consistently, BBR conferred similar antioxidative and anti-inflammatory effects against simulated ischemia reperfusion injury in cultured H9C2 cardiomyocytes. SIRT1 siRNA administration also abolished these effects. In summary, our results demonstrate that BBR significantly improves post-MI/R cardiac function recovery and reduces infarct size against MI/R injury possibly due to its strong antioxidative and anti-inflammatory activity. Additionally, SIRT1 signaling plays a key role in this process.

PMID:
26788242
PMCID:
PMC4691633
DOI:
10.1155/2016/1689602
[PubMed - indexed for MEDLINE]
Free PMC Article
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