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Biosensors (Basel). 2016 Jan 15;6(1). pii: E2. doi: 10.3390/bios6010002.

Cancer-Cells on Chip for Label-Free Detection of Secreted Molecules.

Author information

1
Laboratoire de Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires-Institut de Chimie et Biochimie Moléculaires et Supramoléculaires-Université Claude Bernard Lyon 1, Villeurbanne 69100, France. berthuy@scienion.de.
2
Laboratoire de Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires-Institut de Chimie et Biochimie Moléculaires et Supramoléculaires-Université Claude Bernard Lyon 1, Villeurbanne 69100, France. loic.blum@univ-lyon1.fr.
3
Laboratoire de Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires-Institut de Chimie et Biochimie Moléculaires et Supramoléculaires-Université Claude Bernard Lyon 1, Villeurbanne 69100, France. christophe.marquette@univ-lyon1.fr.

Abstract

In the present report, we are making the proof of concept of cell small populations (from 1 to 100 cells) spotting, culture and secretion detection on a gold surface. In order to keep the cells in a hydrated environment during the robotized micropipetting and to address different cell lines on a single chip, a biocompatible alginate polymer was used. This approach enables the encapsulation of the cell in a very small volume (30 nL), directly on the substrate and permits a precise control of the number of cells in each alginate bead. After 24 h of culture, the adherent cells are ready for surface plasmon resonance imaging (SPRi) experimentation. To enable the detection of secreted proteins, various antibodies are immobilized in an organized manner on a SPRi sensor and permitted the multiplex detection of different proteins secreted by the different cultured cell lines. Evidence of the real-time detection will be presented for Prostate Specific Antigen (PSA) and β-2-microglobulin (B2M) secreted by prostate cancer cells following induction by dihydrotestosterone (DHT). Different kinetics for the two secreted proteins were then demonstrated and precisely determined using the chip.

KEYWORDS:

SPRi; biosensor; cell encapsulation; cell microarray; real-time

PMID:
26784243
PMCID:
PMC4810394
DOI:
10.3390/bios6010002
[Indexed for MEDLINE]
Free PMC Article

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