Format

Send to

Choose Destination
Biomaterials. 2016 Mar;83:249-56. doi: 10.1016/j.biomaterials.2016.01.026. Epub 2016 Jan 11.

The effect of surface modification of mesoporous silica micro-rod scaffold on immune cell activation and infiltration.

Author information

1
John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138, USA; The Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA.
2
School of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, South Korea.
3
John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138, USA; The Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA. Electronic address: mooneyd@seas.harvard.edu.

Abstract

Biomaterial scaffold based vaccines show significant potential in generating potent antigen-specific immunity. However, the role of the scaffold surface chemistry in initiating and modulating the immune response is not well understood. In this study, a mesoporous silica micro-rod (MSR) scaffold was modified with PEG, PEG-RGD and PEG-RDG groups. PEG modification significantly enhanced BMDC activation marker up-regulation and IL-1β production in vitro, and innate immune cell infiltration in vivo. PEG-RGD MSRs and PEG-RDG MSRs displayed decreased inflammation compared to PEG MSRs, and the effect was not RGD specific. Finally, the Nlrp3 inflammasome was found to be necessary for MSR stimulated IL-1β production in vitro and played a key role in regulating immune cell infiltration in vivo. These findings suggest that simply modulating the surface chemistry of a scaffold can regulate its immune cell infiltration profile and have implications for the design and development of new material based vaccines.

KEYWORDS:

Acute inflammation; Inflammasome; Mesoporous silica; Poly(ethylene glycol); RGD

PMID:
26784009
PMCID:
PMC4754159
[Available on 2017-03-01]
DOI:
10.1016/j.biomaterials.2016.01.026
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center