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Inflammation. 2016 Apr;39(2):752-8. doi: 10.1007/s10753-015-0302-2.

Protective Effects of Apigenin Against Paraquat-Induced Acute Lung Injury in Mice.

Author information

1
Department of Pharmacy, Yuhuangding Hospital Affiliated to Qingdao University, Yantai, 264000, China.
2
Department of Radiology, Linyi People's Hospital, Linyi, 276000, China.
3
Department of Emergency, Haiyang People's Hospital, No. 73 Haiyang Road, Haiyang, 265100, China. luanruiling@hotmail.com.

Abstract

This study aimed to investigate the protective effects of apigenin against paraquat (PQ)-induced acute lung injury (ALI) in mice. Male Kunming mice were randomly divided into five groups: group 1 (control), group 2 (PQ), group 3 (PQ + apigenin 25 mg/kg), group 4 (PQ + apigenin 50 mg/kg), and group 5 (PQ + apigenin 100 mg/kg). The PQ + apigenin group received apigenin by gavage daily for consecutive 7 days, respectively, while the mice in control and PQ groups were given an equivalent volume of saline. We detected the lung wet/dry weight ratios and the histopathology of the lung. The levels of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), malondialdehyde (MDA), myeloperoxidase (MPO), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) were determined using enzyme-linked immunosorbent assay (ELISA) kits. The activity of nuclear factor (NF)-κB was also determined. The results indicated that apigenin administration decreased biochemical parameters of inflammation and oxidative stress, and improved oxygenation and lung edema in a dose-dependent manner. These protective effects of apigenin were associated with inhibition of NF-κB. In conclusion, apigenin reduces PQ-induced ALI by inhibition of inflammation and oxidative stress.

KEYWORDS:

acute lung injury; apigenin; inflammation; oxidative stress; paraquat

PMID:
26782361
DOI:
10.1007/s10753-015-0302-2
[Indexed for MEDLINE]

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