(a−f) Descriptions of six largest classes of TMH trimer structure with specific sequence motifs enriched at the interhelical interface. Sequence motifs are constituted by pairs of amino acids belonging to three chemical classes (G/A/S, small: glycine, alanine or serine; I/L/V/M, large: isoleucine, leucine, valine or methionine; F/W/Y or aromatic: phenylalanine, tryptophan or tyrosine). Residues in the motif are aligned along an ideal helix following their sequence separation (register). The helix number describes on which helix or helices of a reference trimer are found the motifs. For example, if a motif is found on helices 1 and 2 in a given trimer, the corresponding helix numbers are given as: ½. The topology and helix number of the reference trimer for each class are described in . Each helix is colored using a blue to red spectrum from N to Cterminus. The reported motifs were found in at least 20% of trimer interacting regions in a given class and are ordered from left to right according to their frequency of occurrence in that class.

(a−d) Consensus interaction networks created by each of the two sequence motif residues enriched at specific TMH trimer structure class labeled in the figure. A dashed red line indicates a consensus interhelical interaction between a motif residue (magenta) and a residue on an adjacent helix (cyan). Interaction networks are highlighted for a given sequence motif in three functionally unrelated membrane proteins.

(a−f) Enriched sequence motifs create unique trimer-specific interatomic van der Waals (black dotted line) and hydrogen bonding (red dotted line) interactions in local regions of the trimers defined by red and blue planes at the center of each panel. Interatomic interactions are defined for any pair of atoms distant by <5 Å belonging to one of the two motif residues (magenta) and a residue on an adjacent helix. The red and blue boxes highlight the atomic contacts from the corresponding colored planes created by each residue of the following motifs and trimer structure classes: (G/A/S)-X₃-(G/A/S) motif specific to all right-handed trimers (a); (I/L/V/M)-X₃-(G/A/S) motif specific to all left-handed trimers (b); (G/A/S)-X₂-(F/W/Y/(M)) motif specific to parallel and left-handed trimers (c); (I/L/V/M)-X₃-(F/W/Y) motif specific to parallel and right-handed trimers (d); (F/W/Y)-X₃-(G/A/S) motif specific to left- and right-handed trimers (e); (F/W/Y)-X₆-(G/A/S) motif specific to left- and right-handed IItrimers (f). *, methionine also found in the second position with similar contacts; +, histidine also found in the first position with similar contacts.

(a) Distribution of residue pairs as a function of their coevolutionary strengths measured by the method EVfold23. The coevolutionary strength of a pair is reported relative to the distribution of coevolutionary scores for all residue pairs as a rank (the lower the rank, the higher the relative coevolutionary strength). Selected residue pairs are in contact across the trimer interhelical interface and involve motif residues (black) or do not involve motif residues (gray). The Z score describes the statistical significance of the difference in rank distribution between the two classes of residue pairs. (b) Example of coevolutionary scores (direct interaction (DI) score) for interresidue contacts involving either a residue in a motif (magenta) or the same residue type not in a motif (cyan) at a trimer interface. (c) Comparison between the energy contribution to the trimer stability of a residue in a motif (black) and that of the same residue type not in a motif (gray). The energy contribution was calculated by alanine scanning, and the comparison was performed for ten different motif residues and reported as mean values + s.d. *P < 0.01, comparison between motif and nonmotif residues’ energetic contributions (Welch’s two-sided t-test). (d) Example of interaction energy between contacts involving a motif residue (magenta) and contacts not involving motif residues (cyan) at a trimer interface.

(a) Distribution of trimer unit structural changes (measured by C_α r.m.s. deviation in Å) in multipass membrane proteins crystallized in distinct conformations. The statistical significance in the distribution of C_α r.m.s. deviation between trimers bearing or not sequence−3Dcontact motifs was calculated using Welch’s two-sided t-test. (b−d) Examples of multipass membrane protein X-ray crystallography structures crystallized in two distinct conformations (superimposed backbone representations in blue and yellow). The trimer units containing a sequence-contact motif (red window) do not change conformations.