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Int J Vitam Nutr Res. 2015;85(3-4):174-84. doi: 10.1024/0300-9831/a000236.

Combined 1,25-Dihydroxy-vitamin D and Resveratrol: A Novel Therapeutic Approach to Ameliorate Ischemia Reperfusion-Induced Myocardial Injury.

Author information

1
1 Herbal Medicine Research Center, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
2
2 Department of Physiology, School of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
3
3 Department of Physiology, International Campus, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
4
4 Department of Physiology, Arsanjan Branch, Islamic Azad University, Fars, Iran.
5
5 , Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
6
6 Department of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.

Abstract

The aim of this study was to assess the effect of combined 1,25-dihydroxyvitamin D (1,25 D) and resveratrol on cardiac arrhythmias, infarct size, and transcription of catalase, thioredoxin-1 and B-cell lymphoma 2 (Bcl-2), following myocardial ischemia-reperfusion (IR) in male rats. Ligation of coronary artery was performed in rats (n = 6 per group) without any treatment (IR group), pretreated with 0.1 μg/kg/day of 1,25 D (1,25 D + IR), 1 mg/kg/day of resveratrol (Res + IR) or a combination (1,25 D + Res + IR) for 14 days. Arrhythmias were analyzed according to the Lambeth conventions, and infarct size was measured by 2,3,5-triphenyl-2H-tetrazolium chloride staining. Expression of prosurvival genes was evaluated by real-time polymerase chain reaction. In the 1,25 D + Res + IR group the mean infarct size was 17.6 ± 3.5 %, which was significantly less than that in the IR, 1,25 D + IR, and Res + IR groups (p < 0.001). Although the single therapy of either 1,25 D or resveratrol did not change the incidence of arrhythmias significantly, a reduction in the number of ventricular ectopic beats was noted in group 1,25 D + Res + IR (179.19 ± 58.87, p < 0.001 vs IR; p < 0.05 vs Res + IR; p < 0.01 vs Vit D + IR). Combination of 1,25 D and resveratrol increased transcription of catalase by 119 ± 37 % (p < 0.001 vs IR, p < 0.01 vs Res + IR, p < 0.001 vs 1,25 D + IR). Our study showed that combination of a non-hypotensive dose of 1,25 D and resveratrol can be a novel and effective strategy for protecting against ischemia.

KEYWORDS:

1,25-Dihydroxyvitamin D; catalase; myocardial ischemia-reperfusion; resveratrol

PMID:
26780396
DOI:
10.1024/0300-9831/a000236
[Indexed for MEDLINE]

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