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Nat Med. 2016 Feb;22(2):154-62. doi: 10.1038/nm.4035. Epub 2016 Jan 18.

Targeting of the pulmonary capillary vascular niche promotes lung alveolar repair and ameliorates fibrosis.

Cao Z1,2,3, Lis R1,2, Ginsberg M4, Chavez D1,5, Shido K1,2, Rabbany SY1,2,6, Fong GH7, Sakmar TP8,9, Rafii S1,2, Ding BS1,3,5.

Author information

1
Ansary Stem Cell Institute, Weill Cornell Medicine, New York, New York, USA.
2
Division of Regenerative Medicine, Department of Medicine, Weill Cornell Medicine, New York, New York, USA.
3
Laboratory of Birth Defects and Related Diseases of Women and Children, State Key Laboratory of Biotherapy, West China Second University Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, China.
4
Angiocrine Bioscience, New York, New York, USA.
5
Department of Genetic Medicine, Weill Cornell Medicine, New York, New York, USA.
6
Bioengineering Program, Hofstra University, Hempstead, New York, USA.
7
Department of Cell Biology, University of Connecticut, Farmington, Connecticut, USA.
8
Laboratory of Chemical Biology &Signal Transduction, Rockefeller University, New York, New York, USA.
9
Division of Neurogeriatrics, Center for Alzheimer Research, Karolinska Institutet, Huddinge, Sweden.

Abstract

Although the lung can undergo self-repair after injury, fibrosis in chronically injured or diseased lungs can occur at the expense of regeneration. Here we study how a hematopoietic-vascular niche regulates alveolar repair and lung fibrosis. Using intratracheal injection of bleomycin or hydrochloric acid in mice, we show that repetitive lung injury activates pulmonary capillary endothelial cells (PCECs) and perivascular macrophages, impeding alveolar repair and promoting fibrosis. Whereas the chemokine receptor CXCR7, expressed on PCECs, acts to prevent epithelial damage and ameliorate fibrosis after a single round of treatment with bleomycin or hydrochloric acid, repeated injury leads to suppression of CXCR7 expression and recruitment of vascular endothelial growth factor receptor 1 (VEGFR1)-expressing perivascular macrophages. This recruitment stimulates Wnt/β-catenin-dependent persistent upregulation of the Notch ligand Jagged1 (encoded by Jag1) in PCECs, which in turn stimulates exuberant Notch signaling in perivascular fibroblasts and enhances fibrosis. Administration of a CXCR7 agonist or PCEC-targeted Jag1 shRNA after lung injury promotes alveolar repair and reduces fibrosis. Thus, targeting of a maladapted hematopoietic-vascular niche, in which macrophages, PCECs and perivascular fibroblasts interact, may help to develop therapy to spur lung regeneration and alleviate fibrosis.

Comment in

PMID:
26779814
PMCID:
PMC4872630
DOI:
10.1038/nm.4035
[Indexed for MEDLINE]
Free PMC Article

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