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J Periodontol. 2016 May;87(5):583-90. doi: 10.1902/jop.2016.150538. Epub 2016 Jan 16.

Collagen Membranes Adsorb the Transforming Growth Factor-β Receptor I Kinase-Dependent Activity of Enamel Matrix Derivative.

Author information

1
Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland.
2
Department of Preventive, Restorative and Pediatric Dentistry, School of Dental Medicine, University of Bern.
3
Robert K. Schenk Laboratory of Oral Histology, School of Dental Medicine, University of Bern.
4
Department of Oral Biology, Medical University of Vienna, Vienna, Austria.

Abstract

BACKGROUND:

Enamel matrix derivative (EMD) and collagen membranes (CMs) are simultaneously applied in regenerative periodontal surgery. The aim of this study is to evaluate the ability of two CMs and a collagen matrix to adsorb the activity intrinsic to EMD that provokes transforming growth factor (TGF)-β signaling in oral fibroblasts.

METHODS:

Three commercially available collagen products were exposed to EMD or recombinant TGF-β1, followed by vigorous washing. Oral fibroblasts were either seeded directly onto collagen products or were incubated with the respective supernatant. Expression of TGF-β target genes interleukin (IL)-11 and proteoglycan 4 (PRG4) was evaluated by real time polymerase chain reaction. Proteomic analysis was used to study the fraction of EMD proteins binding to collagen.

RESULTS:

EMD or TGF-β1 provoked a significant increase of IL-11 and PRG4 expression of oral fibroblasts when seeded onto collagen products and when incubated with the respective supernatant. Gene expression was blocked by the TGF-β receptor I kinase inhibitor SB431542. Amelogenin bound most abundantly to gelatin-coated culture dishes. However, incubation of palatal fibroblasts with recombinant amelogenin did not alter expression of IL-11 and PRG4.

CONCLUSION:

These in vitro findings suggest that collagen products adsorb a TGF-β receptor I kinase-dependent activity of EMD and make it available for potential target cells.

KEYWORDS:

Collagen; gene expression; guided tissue regeneration; interleukin-11; proteoglycans; transforming growth factor beta1

PMID:
26777762
DOI:
10.1902/jop.2016.150538
[Indexed for MEDLINE]

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