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Food Chem. 2016 May 15;199:509-15. doi: 10.1016/j.foodchem.2015.12.041. Epub 2015 Dec 10.

Agaricus bisporus and its in vitro culture as a source of indole compounds released into artificial digestive juices.

Author information

1
Department of Pharmaceutical Botany, Jagiellonian University, Collegium Medicum, Medyczna St 9, Kraków 30-688, Poland. Electronic address: muchon@poczta.fm.
2
Department of Pharmaceutical Botany, Jagiellonian University, Collegium Medicum, Medyczna St 9, Kraków 30-688, Poland.
3
Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Kraków 30-059, Poland.
4
Department of Inorganic and Analytical Chemistry, Jagiellonian University, Collegium Medicum, Medyczna St 9, 30-688 Kraków, Poland.

Abstract

The popularity of Agaricus bisporus results not only from the quality of the flavors, but also from the content of many substances of therapeutic properties. This paper presents a study on RP-HPLC determination of the content of indole compounds released from the lyophilized biomass from in vitro cultures of A. bisporus into artificial digestive juices at 37°C. A. bisporus in vitro cultures were grown on media enriched with zinc salts. The release of 5-hydroxy-l-tryptophan and l-tryptophan was found in the greatest number of samples. The content of 5-hydroxy-l-tryptophan in the investigated samples ranged from 86.62 to 531 mg/100g d.w. The amount of l-tryptophan was determined within the range of 1.63-4.68 mg/100g d.w. and for melatonin 0.43-0.64 mg/100g d.w. It was demonstrated for the first time that in vitro cultures of A. bisporus release indole compounds in conditions simulating the human digestive tract.

KEYWORDS:

Agaricus bisporus; Artificial gastric juices; In vitro culture; Indole compounds; Zinc

PMID:
26776002
DOI:
10.1016/j.foodchem.2015.12.041
[Indexed for MEDLINE]

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