Comparison of multiple-locus variable-number tandem-repeat analysis with pulsed-field gel electrophoresis typing of carbapenemases producing Acinetobacter baumannii isolated from burn patients

Leila Azimi; Malihe Talebi; Farzaneh Khodaei; Mohammad Najafi; Abdolaziz Rastegar Lari

doi:10.1016/j.burns.2015.08.024

Comparison of multiple-locus variable-number tandem-repeat analysis with pulsed-field gel electrophoresis typing of carbapenemases producing Acinetobacter baumannii isolated from burn patients

Burns. 2016 Mar;42(2):441-5. doi: 10.1016/j.burns.2015.08.024. Epub 2016 Jan 13.

Authors

Leila Azimi¹, Malihe Talebi², Farzaneh Khodaei³, Mohammad Najafi⁴, Abdolaziz Rastegar Lari⁵

Affiliations

¹ Pediatric Infectious Research Center, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
² Department of Microbiology, Iran University of Medical Sciences, Tehran, Iran.
³ Department of Microbiology, Tabriz University of Medical Sciences, Tabriz, Iran.
⁴ Department of Biochemistry, Iran University of Medical Sciences, Tehran, Iran.
⁵ Pediatric Infectious Research Center, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Microbiology, Iran University of Medical Sciences, Tehran, Iran. Electronic address: azizlari@gmail.com.

PMID: 26774604
DOI: 10.1016/j.burns.2015.08.024

Abstract

Background: Acinetobacter baumannii is one of the important causes of nosocomial infection especially in burn patients. So, carbapenemase producing strains can make serious therapeutic problems. Molecular epidemiology studies play key role in decreasing the incidence of carbapenemase producing strains.

Methods: In this study, 50 carbapenem resistant A. baumannii isolates from burn patients were collected. Antibiotic susceptibility testing and PCR assay was performed for detection of blaVIM, blaIMP, blaOXA-23,blaOXA-48,blaNDM-1, blaSPM-1, blaOxa-51 and blaKPC genes. Molecular typing for carbapenemase producing strains were performed by PFGE and MLVA.

Results: According to the results of antibiotic susceptibility tests all of strains were XDR and 46 (92%) harbored carbapenemase genes. PFGE and MLVA results showed the presence of 11 and 5 dominant clonal types respectively.

Conclusion: The results of this study were showed the presence of certain clonal groups in two different wards of hospital indicating the spread of carbapenemase producing A. baumannii. On the other hand, the results showed the more discriminating power for PFGE.

Keywords: A. baumannii; MLVA; PFGE.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Acinetobacter baumannii / genetics*
Bacterial Proteins / genetics
Bacterial Typing Techniques / methods*
Burns / microbiology*
Drug Resistance, Bacterial / genetics
Drug Resistance, Multiple, Bacterial / genetics
Electrophoresis, Gel, Pulsed-Field / methods*
Humans
Microbial Sensitivity Tests
Minisatellite Repeats / genetics*
Molecular Epidemiology
Polymerase Chain Reaction
beta-Lactamases / genetics

Substances

Bacterial Proteins
beta-Lactamases
carbapenemase