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Virology. 2016 Feb;489:243-51. doi: 10.1016/j.virol.2015.12.012. Epub 2016 Jan 14.

Genomic characterization and comparison of seven Myoviridae bacteriophage infecting Bacillus thuringiensis.

Author information

1
Department of Biology, James Madison University, Harrisonburg, VA, USA.
2
Department of Biology, James Madison University, Harrisonburg, VA, USA. Electronic address: quinnmr@dukes.jmu.edu.
3
Department of Biology, James Madison University, Harrisonburg, VA, USA. Electronic address: brouilak@dukes.jmu.edu.
4
Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, MD, USA. Electronic address: scaruso@umbc.edu.
5
Department of Biology, James Madison University, Harrisonburg, VA, USA. Electronic address: cresawsg@jmu.edu.
6
Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, MD, USA. Electronic address: erill@umbc.edu.
7
Department of Biological Sciences, University of Mary Washington, Fredericksburg, VA, USA. Electronic address: llewis@umw.edu.
8
Department of Biological Sciences, University of Mary Washington, Fredericksburg, VA, USA. Electronic address: kloesser@umw.edu.
9
Department of Biology, James Madison University, Harrisonburg, VA, USA. Electronic address: patemt@dukes.jmu.edu.
10
Department of Integrated Sciences & Technology, James Madison University, Harrisonburg, VA, USA. Electronic address: scottcm@jmu.edu.
11
Department of Integrated Sciences & Technology, James Madison University, Harrisonburg, VA, USA. Electronic address: stockwsb@jmu.edu.
12
Department of Integrated Sciences & Technology, James Madison University, Harrisonburg, VA, USA. Electronic address: templelm@jmu.edu.

Abstract

Bacillus thuringiensis Kurstaki, a bacterium that is a source of biopesticides and a safe simulant for pathogenic Bacillus species, was used to isolate seven unique bacteriophages. The phage genomes were sequenced and ranged in size from 158,100 to 163,019 bp encoding 290-299 genes, and the GC content of ~38% was similar to that of the host bacterium. All phages had terminal repeats 2-3 kb long. Three of the phages encoded tRNAs and three contained a self-splicing intron in the DNA polymerase gene. They were categorized as a single cluster (>60% nucleotide conservation) containing three subclusters (>80% nucleotide conservation), supported by genomic synteny and phylogenetic analysis. Considering the published genomes of phages that infect the genus Bacillus and noting the ability of many of the Bacillus cereus group phages to infect multiple species, a clustering system based on gene content is proposed.

KEYWORDS:

Bacillus thuringiensis; Bacteriophages; Bioinformatics; Genomics; Phages; Sequencing

PMID:
26773385
DOI:
10.1016/j.virol.2015.12.012
[Indexed for MEDLINE]
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