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Gene. 2016 Apr 15;580(2):104-110. doi: 10.1016/j.gene.2016.01.005. Epub 2016 Jan 7.

Molecular cloning of the sex-related gene PSI in Bemisia tabaci and its alternative splicing properties.

Author information

1
College of Plant Protection of Hunan Agricultural University, Changsha 410128, PR China; Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China. Electronic address: liuyating19900209@163.com.
2
Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China. Electronic address: xiewen@caas.cn.
3
Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China. Electronic address: xinyang3856@163.com.
4
Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China. Electronic address: glt0629@163.com.
5
Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China. Electronic address: wangshaoli@caas.cn.
6
Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China. Electronic address: wuqingjun@caas.cn.
7
Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China. Electronic address: yyll88523@126.com.
8
Department of Entomology, University of Kentucky, Lexington, KY 40546-0091, USA. Electronic address: xuguozhou@uky.edu.
9
Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China. Electronic address: zhangyoujun@caas.cn.

Abstract

The P-element somatic inhibitor (PSI) is gene known to regulate the transcription of doublesex (dsx) when transformer (tra) is absent in Bombyx mori. In this study, we identified and characterized a PSI homolog in Bemisia tabaci (BtPSI). BtPSI cDNA had a total length of 5700 bp and contained a predicted open reading frame (ORF) of 2208 nucleotides encoding for 735 amino acids. Multiple sequence alignments of the common regions of PSI proteins from B. tabaci and five other insect species revealed a high degree of sequence conservation. BtPSI is expressed in all stages of B. tabaci development, and expression did not significantly differ between female and male adult. A total of 92 BtPSI isoforms (78 in female and 22 in male) were identified, and a marker indicating the female-specific form was found. These results increase the understanding of genes that may determine sex in B. tabaci and provide a foundation for research on the sex determination mechanism in this insect.

KEYWORDS:

Alternative splicing; Bemisia tabaci; Haplodiploidy; PSI; Sex determination

PMID:
26773355
DOI:
10.1016/j.gene.2016.01.005
[Indexed for MEDLINE]

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