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Int J Clin Exp Med. 2015 Oct 15;8(10):17773-80. eCollection 2015.

The protective effects of Masson pine pollen aqueous extract on CCl4-induced oxidative damage of human hepatic cells.

Author information

1
International Center for Liver Disease Treatment, 302 Hospital Beijing 10039, China.
2
Institute of Health and Environmental Medicine Tianjin 30050, China.
3
Department of Nutrition, General Hospital of P.L.A Beijing 100853, China.
4
The Second Artillery General Hospital of P.L.A Beijing 100082, China.
5
Animal Center, Beijing 302 Hospital Beijing 100039, China.

Abstract

OBJECTIVE:

We observed the effects of Masson pine pollen aqueous extracts (MPPAE) on CCl4-induced oxidative damage of the human hepatic cell line L-02.

METHODS:

We created an in vitro model of oxidative liver damage by treating L-02 human hepatic cells with 40 mmol/L CCl4. Effects of different concentrations of MPPAE on cell proliferation, morphology, and change of functional indexes were observed after addition of CCl4.

RESULTS:

CCl4 was toxic to proliferation, cell morphology, and functionality of hepatic cells. It decreased proliferation by 29.3-38.4% and increased AST and ALT activities by 22.3% and 99.2%, respectively. The oxidative stress also disrupted hepatic cell growth and induced pyknosis. Although MPPAE did not prevent decreased proliferation of L-02 cells, the treatment alleviated some CCl4-induced cell morphology changes and inhibited the abnormal rise of ALT (39.8%-70.1%) and AST (14.75-27.25%) activities in a dose dependent manner. A high dose of MPPAE (400 mg/L) ameliorated nucleus deformation to an almost normal appearance.

CONCLUSIONS:

According to our in vitro model, MPPAE specifically prevented the changes in cell morphology and functional injury caused by CCL4 treatment; however, it offered limited protection against damage-induced reduction of proliferation.

KEYWORDS:

Aqueous extract; CCl4; hepatic cells; masson pine pollen; oxidative damage

PMID:
26770368
PMCID:
PMC4694268

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