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J Invest Dermatol. 2016 Apr;136(4):788-97. doi: 10.1016/j.jid.2015.12.027. Epub 2016 Jan 6.

MALT1 Protease Activity Controls the Expression of Inflammatory Genes in Keratinocytes upon Zymosan Stimulation.

Author information

1
Interfaculty Institute for Biochemistry, Eberhard Karls University of Tuebingen, Tuebingen, Germany.
2
Department of Dermatology, Eberhard Karls University of Tuebingen, Tuebingen, Germany.
3
Institute of Medical Genetics and Applied Genomics, Eberhard Karls University of Tuebingen, Tuebingen, Germany.
4
Interfaculty Institute for Biochemistry, Eberhard Karls University of Tuebingen, Tuebingen, Germany. Electronic address: Stephan.Hailfinger@uni-tuebingen.de.

Abstract

The protease activity of the paracaspase mucosa-associated lymphoid tissue lymphoma translocation gene 1 (MALT1) plays an important role in antigen receptor-mediated lymphocyte activation by controlling the activity of the transcription factor nuclear factor-κB and is thus essential for the expression of inflammatory target genes. MALT1 is not only present in cells of the hematopoietic lineage, but is ubiquitously expressed. Here we report that stimulation with zymosan or Staphylococcus aureus induced MALT1 protease activity in human primary keratinocytes. Inhibition of the Src family of kinases or novel protein kinase C isoforms as well as silencing of CARMA2 or BCL10 interfered with activation of MALT1 protease. Silencing or inhibition of MALT1 protease strongly decreased the expression of important inflammatory genes such as TNFα, IL-17C, CXCL8 and HBD-2. MALT1-inhibited cells were unable to mount an antimicrobial response upon zymosan stimulation or phorbolester/ionomycin treatment, demonstrating a central role of MALT1 protease activity in keratinocyte immunity and suggesting MALT1 as a potential target in inflammatory skin diseases.

PMID:
26767426
DOI:
10.1016/j.jid.2015.12.027
[Indexed for MEDLINE]
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