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J Invertebr Pathol. 2016 Feb;134:23-26. doi: 10.1016/j.jip.2016.01.003. Epub 2016 Jan 5.

A formulated double-stranded RNA diet for reducing Penaeus monodon densovirus infection in black tiger shrimp.

Author information

1
Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand.
2
Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand; Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
3
Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand. Electronic address: pongsopee.att@mahidol.ac.th.

Abstract

Penaeus monodon densovirus (PmDNV) is one of the major causes of stunted shrimp in the aquaculture industry in Thailand. Significant reductions in levels of PmDNV as assessed by PCR analysis of shrimp hepatopancreas were seen in both prophylactic and curative experiments after feeding shrimp with a formulated diet containing mixed inactivated bacteria harboring dsRNAs corresponding to the PmDNV ns1 and vp genes. Significant reductions of approximately 88% (prophylactic) and 64% (curative) of PmDNV were observed, suggesting that this diet has a high potential for application in commercial aquaculture for reducing PmDNV associated stunted growth of shrimp.

KEYWORDS:

Double-stranded RNA; Oral delivery; Penaeus monodon densovirus; PmDNV inhibition; RNAi; Shrimp

PMID:
26767321
DOI:
10.1016/j.jip.2016.01.003
[Indexed for MEDLINE]

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