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Dev Cell. 2016 Jan 11;36(1):63-78. doi: 10.1016/j.devcel.2015.12.017.

Ubiquitin Receptor Protein UBASH3B Drives Aurora B Recruitment to Mitotic Microtubules.

Author information

1
Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Centre National de la Recherche Scientifique UMR 7104, Institut National de la Santé et de la Recherche Médicale U964, Université de Strasbourg, 67404 Illkirch, France.
2
Institute for Genetics, University of Cologne, 50674 Cologne, Germany.
3
Université de Strasbourg, Fédération de Médecine Translationnelle de Strasbourg, 67085 Strasbourg, France.
4
Centre for Gene Regulation & Expression, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.
5
Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Centre National de la Recherche Scientifique UMR 7104, Institut National de la Santé et de la Recherche Médicale U964, Université de Strasbourg, 67404 Illkirch, France; Computer Science Department, ICube, UMR 7357, University of Strasbourg, CNRS, Fédération de Médecine Translationnelle, 67085 Strasbourg, France.
6
Computer Science Department, ICube, UMR 7357, University of Strasbourg, CNRS, Fédération de Médecine Translationnelle, 67085 Strasbourg, France.
7
Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Centre National de la Recherche Scientifique UMR 7104, Institut National de la Santé et de la Recherche Médicale U964, Université de Strasbourg, 67404 Illkirch, France. Electronic address: sumara@igbmc.fr.

Abstract

Mitosis ensures equal segregation of the genome and is controlled by a variety of ubiquitylation signals on substrate proteins. However, it remains unexplored how the versatile ubiquitin code is read out during mitotic progression. Here, we identify the ubiquitin receptor protein UBASH3B as an important regulator of mitosis. UBASH3B interacts with ubiquitylated Aurora B, one of the main kinases regulating chromosome segregation, and controls its subcellular localization but not protein levels. UBASH3B is a limiting factor in this pathway and is sufficient to localize Aurora B to microtubules prior to anaphase. Importantly, targeting Aurora B to microtubules by UBASH3B is necessary for the timing and fidelity of chromosome segregation in human cells. Our findings uncover an important mechanism defining how ubiquitin attachment to a substrate protein is decoded during mitosis.

KEYWORDS:

Aurora B; UBASH3B; chromosome segregation; mitosis; ubiquitin; ubiquitin receptor

PMID:
26766443
PMCID:
PMC5400057
DOI:
10.1016/j.devcel.2015.12.017
[Indexed for MEDLINE]
Free PMC Article

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