Format

Send to

Choose Destination
Mol Biol Cell. 2016 Mar 1;27(5):812-27. doi: 10.1091/mbc.E15-02-0101. Epub 2016 Jan 13.

Augmin shapes the anaphase spindle for efficient cytokinetic furrow ingression and abscission.

Author information

1
Creative Research Institution, Hokkaido University, Sapporo 001-0021, Japan Department of Life Sciences, School of Arts and Sciences, University of Tokyo, Tokyo 153-8902, Japan Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan ruehara@sci.hokudai.ac.jp.
2
Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan.
3
Creative Research Institution, Hokkaido University, Sapporo 001-0021, Japan.
4
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
5
Department of Life Sciences, School of Arts and Sciences, University of Tokyo, Tokyo 153-8902, Japan.
6
Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna Biocenter Campus, 1030 Vienna, Austria.

Abstract

During anaphase, distinct populations of microtubules (MTs) form by either centrosome-dependent or augmin-dependent nucleation. It remains largely unknown whether these different MT populations contribute distinct functions to cytokinesis. Here we show that augmin-dependent MTs are required for the progression of both furrow ingression and abscission. Augmin depletion reduced the accumulation of anillin, a contractile ring regulator at the cell equator, yet centrosomal MTs were sufficient to mediate RhoA activation at the furrow. This defect in contractile ring organization, combined with incomplete spindle pole separation during anaphase, led to impaired furrow ingression. During the late stages of cytokinesis, astral MTs formed bundles in the intercellular bridge, but these failed to assemble a focused midbody structure and did not establish tight linkage to the plasma membrane, resulting in furrow regression. Thus augmin-dependent acentrosomal MTs and centrosomal MTs contribute to nonredundant targeting mechanisms of different cytokinesis factors, which are required for the formation of a functional contractile ring and midbody.

PMID:
26764096
PMCID:
PMC4803307
DOI:
10.1091/mbc.E15-02-0101
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Atypon Icon for PubMed Central
Loading ...
Support Center