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Phytother Res. 2016 Mar;30(3):510-6. doi: 10.1002/ptr.5558. Epub 2016 Jan 13.

New Findings on the Effects of Tannic Acid: Inhibition of L-Type Calcium Channels, Calcium Transient and Contractility in Rat Ventricular Myocytes.

Author information

1
Hebei Medical University, 361 East Zhongshan Road, Shijiazhuang, 050017, Hebei, China.
2
The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, Hebei, China.
3
Department of Pharmacology, Hebei University of Chinese Medicine, Shijiazhuang, 050200, Hebei, China.
4
Department of Medicinal Chemistry, Hebei University of Chinese Medicine, Shijiazhuang, 050200, Hebei, China.
5
Department of Infectious Diseases, Hebei General Hospital, No. 348, Heping West Road, Shijiazhuang, Hebei, 050051, China.

Abstract

Tannic acid (TA) is a group of water-soluble polyphenolic compounds that occur mainly in plant-derived feeds, food grains and fruits. Many studies have explored its biomedical properties, such as anticancer, antibacterial, antimutagenic, antioxidant, antidiabetic, antiinflammatory and antihypertensive activities. However, the effects of TA on the L-type Ca(2+) current (ICa-L) of cardiomyocytes remain undefined. The present study examined the effects of TA on ICa-L using the whole-cell patch-clamp technique and on intracellular Ca(2+) handling and cell contractility in rat ventricular myocytes with the aid of a video-based edge detection system. Exposure to TA resulted in a concentration- and voltage-dependent blockade of ICa-L, with the half maximal inhibitory concentration of 1.69 μM and the maximal inhibitory effect of 46.15%. Moreover, TA significantly inhibited the amplitude of myocyte shortening and peak value of Ca(2+) transient and increased the time to 10% of the peak. These findings provide new experimental evidence for the cellular mechanism of action of TA and may help to expand clinical treatments for cardiovascular disease.

KEYWORDS:

Ca2+ channel; Ca2+ transient; Tannic acid; cardiomyocyte; myocyte shortening

PMID:
26762248
DOI:
10.1002/ptr.5558
[Indexed for MEDLINE]

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