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Korean J Food Sci Anim Resour. 2015;35(5):707-13. doi: 10.5851/kosfa.2015.35.5.707. Epub 2015 Oct 31.

Whitening Effect of Watersoluble Royal Jelly from South Korea.

Author information

1
Department of Agricultural Biology, National Academy of Agricultural Science, Wanju 55365, Korea.
2
Catholic University of Daegu School of Medicine, Daegu 42472, Korea.
3
School of Biomedical Sciences, Charles Sturt University, Bathurst, NSW 2795, Australia.

Abstract

Royal jelly has been widely used as a health supplement worldwide. However, royal jelly has been implicated in allergic reactions, and we developed a water-soluble royal jelly (WSRJ) without the allergy inducing protein. In this study, we aimed to identify the anti-melanogenic efficacy of WSRJ. B16F1 melanoma cells were first treated with 10 nM α-melanocyte stimulating hormone (α-MSH) and then with various doses of WSRJ. In addition, we investigated the mRNA and protein expression of melanogenesis-related genes such as tyrosinase, tyrosinase related protein-1 (TRP-1) and TRP-2 by reverse transcription-polymerase chain reaction and western blotting. WSRJ directly inhibited tyrosinase and cellular tyrosinase activity, which decreased melanin synthesis in α-MSH stimulated B16F1 melanoma cells a level comparable to that observed with arbutin. WSRJ decreased the mRNA and protein expressions of tyrosinase, TRP-1, and TRP-2, which was comparable to that observed with arbutin. WSRJ has strong anti-melanogenic activity, which invoice direct inhibition of tyrosinase enzyme activity and suppression of expression of melanogenesis related genes. Results from this study suggests that WSRJ is a potential candidate for the treatment of skin pigmentation.

KEYWORDS:

TRP-1; TRP-2; royal jelly; tyrosinase; water-soluble royal jelly; α-MSH

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