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ACS Nano. 2016 Feb 23;10(2):2827-33. doi: 10.1021/acsnano.5b07948. Epub 2016 Jan 22.

Real-Time Capture and Visualization of Individual Viruses in Complex Media.

Author information

1
nanoView Diagnostics Inc. , Boston, Massachusetts 02215, United States.
2
Microbiology Department, Boston University School of Medicine , Boston, Massachusetts 02118, United States.

Abstract

Label-free imaging of individual viruses and nanoparticles directly in complex solutions is important for virology research and biosensing applications. A successful visualization technique should be rapid, sensitive, and inexpensive, while needing minimal sample preparation or user expertise. Current approaches typically require fluorescent labeling or the use of an electron microscope, which are expensive and time-consuming to use. We have developed an imaging technique for real-time, sensitive, and label-free visualization of viruses and nanoparticles directly in complex solutions such as serum. By combining the advantages of a single-particle reflectance imaging sensor, with microfluidics, we perform real-time digital detection of individual 100 nm vesicular stomatitis viruses as they bind to an antibody microarray. Using this approach, we have shown capture and visualization of a recombinant vesicular stomatitis virus Ebola model (rVSV-ZEBOV) at 100 PFU/mL in undiluted fetal bovine serum in less than 30 min.

KEYWORDS:

Ebola; biosensor; interferometry; label-free; single virus

PMID:
26760677
PMCID:
PMC5019356
DOI:
10.1021/acsnano.5b07948
[Indexed for MEDLINE]
Free PMC Article

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