A complete deletion mutant of the Escherichia coli dnaKdnaJ operon

R Ohki; R Morita; T Kawamata; H Uchida; M Ohki

doi:10.1016/0167-4781(89)90085-7

A complete deletion mutant of the Escherichia coli dnaKdnaJ operon

Biochim Biophys Acta. 1989 Sep 21;1009(1):94-8. doi: 10.1016/0167-4781(89)90085-7.

Authors

R Ohki¹, R Morita, T Kawamata, H Uchida, M Ohki

Affiliation

¹ Department of Molecular Biology, School of Health Sciences, Kyorin University, Hachioji, Japan.

PMID: 2675977
DOI: 10.1016/0167-4781(89)90085-7

Abstract

Southern hydridization analyses of genomic DNAs from various dnaJ mutants of Escherichia coli showed that mutant K7052, which has well characterized dnaK706 and dnaJ705 double mutantions, is a deletion mutant. The deletion is about 8.0 kb long and encompasses the whole of the dnaKdnaJ operon.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cloning, Molecular
DNA, Bacterial / genetics*
Deoxyribonuclease BamHI
Deoxyribonuclease EcoRI
Deoxyribonuclease HindIII
Deoxyribonucleases, Type II Site-Specific
Escherichia coli / genetics*
Heat-Shock Proteins / genetics*
Mutation*
Nucleic Acid Hybridization
Operon*
Restriction Mapping

Substances

DNA, Bacterial
Heat-Shock Proteins
Deoxyribonuclease BamHI
Deoxyribonuclease EcoRI
Deoxyribonuclease HindIII
endodeoxyribonuclease PvuI
Deoxyribonucleases, Type II Site-Specific