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Appl Biochem Biotechnol. 2016 Apr;179(1):94-110. doi: 10.1007/s12010-016-1981-3. Epub 2016 Jan 13.

Synthesis and Characterisation of Biocompatible Polymer-Conjugated Magnetic Beads for Enhancement Stability of Urease.

Author information

1
Faculty of Science, Chemistry Department, Muğla Sıtkı Koçman University, Muğla, Turkey. y-ispirli@hotmail.com.
2
Faculty of Science, Chemistry Department, Muğla Sıtkı Koçman University, Muğla, Turkey.

Abstract

We reported natural polymer-conjugated magnetic featured urease systems for removal of urea effectively. The optimum temperature (20-60 °C), optimum pH (3.0-10.0), kinetic parameters, thermal stability (4-70 °C), pH stability (4.0-9.0), operational stability (0-250 min), reusability (18 times) and storage stability (24 weeks) were studied for characterisation of the urease-encapsulated biocompatible polymer-conjugated magnetic beads. Also, the surface groups and chemical structure of the magnetic beads were determined by using attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The all urease-encapsulated magnetic beads protected their stability of 30-45 % relative activity at 70 °C. A significant increase was observed at their pH stability compared with the free urease for both acidic and alkaline medium. Besides this, their repeatability activity were approximately 100 % during 4(th) run. They showed residual activity of 50 % after 16 weeks. The importance of this work is enhancement stability of immobilised urease by biocompatible polymer-conjugated magnetic beads for the industrial application based on removal of urea.

KEYWORDS:

Fe3O4 nanoparticles; Fe[NiFe]O4 nanoparticles; Magnetic polymeric beads; Urease encapsulation; Urease stability

PMID:
26758712
DOI:
10.1007/s12010-016-1981-3
[Indexed for MEDLINE]

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