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Heredity (Edinb). 2016 May;116(5):424-33. doi: 10.1038/hdy.2015.110. Epub 2016 Jan 13.

The fate of W chromosomes in hybrids between wild silkmoths, Samia cynthia ssp.: no role in sex determination and reproduction.

Author information

1
Institute of Entomology, Biology Centre of The Czech Academy of Science, České Budějovice, Czech Republic.
2
Laboratory of Applied Molecular Entomology, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
3
Laboratory of Applied Entomology, Faculty of Agriculture, Iwate University, Morioka, Japan.

Abstract

Moths and butterflies (Lepidoptera) have sex chromosome systems with female heterogamety (WZ/ZZ or derived variants). The maternally inherited W chromosome is known to determine female sex in the silkworm, Bombyx mori. However, little is known about the role of W chromosome in other lepidopteran species. Here we describe two forms of the W chromosome, W and neo-W, that are transmitted to both sexes in offspring of hybrids from reciprocal crosses between subspecies of wild silkmoths, Samia cynthia. We performed crosses between S. c. pryeri (2n=28, WZ/ZZ) and S. c. walkeri (2n=26, neo-Wneo-Z/neo-Zneo-Z) and examined fitness and sex chromosome constitution in their hybrids. The F1 hybrids of both reciprocal crosses had reduced fertility. Fluorescence in situ hybridization revealed not only the expected sex chromosome constitutions in the backcross and F2 hybrids of both sexes but also females without the W (or neo-W) chromosome and males carrying the W (or neo-W) chromosome. Furthermore, crosses between the F2 hybrids revealed no association between the presence or absence of W (or neo-W) chromosome and variations in the hatchability of their eggs. Our results clearly suggest that the W (or neo-W) chromosome of S. cynthia ssp. plays no role in sex determination and reproduction, and thus does not contribute to the formation of reproductive barriers between different subspecies.

PMID:
26758188
PMCID:
PMC4834383
DOI:
10.1038/hdy.2015.110
[Indexed for MEDLINE]
Free PMC Article

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