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Int J Syst Evol Microbiol. 2016 Mar;66(3):1383-1388. doi: 10.1099/ijsem.0.000889. Epub 2016 Jan 8.

Mobilisporobacter senegalensis gen. nov., sp. nov., an anaerobic bacterium isolated from tropical shea cake.

Author information

1
1​ Aix-Marseille Université, Université de Toulon,CNRS/INSU, IRD, UMR 235, MIO, UM110, 163 Avenue de Luminy, Case 925, F-13288, Marseille, Cedex 09,France.
2
2​ Laboratoire de Microbiologie Appliquée et de Génie Industriel, Ecole Supérieure Polytechnique, Université Cheikh Anta Diop,BP 5005 Dakar-Fann, Dakar,Senegal.
3
3​ Laboratoire de Biochimie, Faculté des Sciences et Techniques, Université Cheikh Anta Diop,BP 5005 Dakar-Fann, Dakar,Senegal.

Abstract

A Gram-stain positive, endospore-forming, strictly anaerobic bacterium, designated strain Gal1T, was isolated from shea cake, a waste material from the production of shea butter, originating from Saraya, Senegal. The cells were rod-shaped, slightly curved, and motile with peritrichous flagella. The strain was oxidase-negative and catalase-negative. Growth was observed at temperatures ranging from 15 to 45 °C (optimum 30 °C) and at pH 6.5-9.3 (optimum pH 7.8). The salinity range for growth was 0-3.5 % NaCl (optimum 1 %). Yeast extract was required for growth. Strain Gal1T fermented various carbohydrates such as mannose, mannitol, arabinose, cellobiose, fructose, glucose, maltose, sucrose, trehalose and lactose and the major end-products were ethanol and acetate. The only major cellular fatty acid was C16 : 0 (19.6 %). The DNA base G+C content of strain Gal1T was 33.8 mol%. Analysis of the 16S rRNA gene sequence of the isolate indicated that this strain was related to Mobilitalea sibirica DSM 26468T with 94.27 % similarity, Clostridium populeti ATTC 35295T with 93.94 % similarity, and Clostridium aminovalericum DSM 1283T and Anaerosporobacter mobilis DSM 15930T with 93.63 % similarity. On the basis of phenotypic characteristics, phylogenetic analysis and the results of biochemical and physiological tests, strain Gal1T was clearly distinguished from closely related genera, and strain Gal1T can be assigned to a novel species of a new genus for which the name Mobilisporobacter senegalensis gen. nov., sp. nov. is proposed. The type strain is Gal1T ( = DSM 26537T = JCM 18753T).

PMID:
26755447
DOI:
10.1099/ijsem.0.000889

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