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Biofouling. 2016;32(1):71-9. doi: 10.1080/08927014.2015.1122759.

The cell aggregating propensity of probiotic actinobacterial isolates: isolation and characterization of the aggregation inducing peptide pheromone.

Author information

1
a Centre for Pheromone Technology, Department of Animal Science , Bharathidasan University , Tiruchirappalli , India.
2
b Bioprocess Technology Laboratory, Department of Microbiology , Bharathidasan University , Tiruchirappalli , India.
3
c Department of Botany and Microbiology , College of Science, King Saud University , Riyadh , Saudi Arabia.
4
d Lee Kong Chian School of Medicine, Nanyang Technological University , Singapore.

Abstract

The auto-aggregating ability of a probiotic is a prerequisite for colonization and protection of the gastrointestinal tract, whereas co-aggregation provides a close interaction with pathogenic bacteria. Peptide pheromone mediated signaling has been studied in several systems. However, it has not yet been explored in prokaryotes, especially actinobacteria. Hence, in the present study, the diffusible aggregation promoting factor was purified from the culture supernatant of a potent actinobacterial probiont and characterized using 20 different actinobacterial cultures isolated from the gut region of chicken and goat. The results showed that the pheromone-like compound induces the aggregation propensity of treated isolates. The factor was found to be a heat stable, acidic pH resistant, low molecular weight peptide which enhances the biofilm forming ability of other actinobacterial isolates. The aggregation promoting factor represents a bacterial sex factor (pheromone) and its characterization confirms its usage in the probiotic formulation.

KEYWORDS:

Actinobacteria; Pheromone; aggregation factor; biofilm; probiotic

PMID:
26754920
DOI:
10.1080/08927014.2015.1122759
[Indexed for MEDLINE]

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