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Nat Methods. 2016 Mar;13(3):229-232. doi: 10.1038/nmeth.3728. Epub 2016 Jan 11.

Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity.

Author information

1
European Molecular Biology Laboratory, European Bioinformatics Institute, Hinxton, Cambridge, UK.
2
Epigenetics Programme, Babraham Institute, Cambridge, UK.
3
Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
4
Medical Research Council Functional Genomics Unit, University of Oxford, UK.
5
Bioinformatics Group, Babraham Institute, Cambridge, UK.
6
Department of Human Genetics, Katholieke Universiteit (KU) Leuven, Leuven, Belgium.
#
Contributed equally

Abstract

We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing that allows for the discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcription. Notably, the method revealed previously unrecognized associations between heterogeneously methylated distal regulatory elements and transcription of key pluripotency genes.

Comment in

PMID:
26752769
PMCID:
PMC4770512
DOI:
10.1038/nmeth.3728
[Indexed for MEDLINE]
Free PMC Article

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