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Anal Chem. 2016 Feb 16;88(4):2140-8. doi: 10.1021/acs.analchem.5b03696. Epub 2016 Jan 28.

Noninvasive Detection of Inflammatory Changes in White Adipose Tissue by Label-Free Raman Spectroscopy.

Author information

1
Department of Biochemistry, Weill Cornell Medical College , New York, New York 10065, United States.
2
Department of Medicine, Weill Cornell Medical College , New York, New York 10065, United States.
3
Department of Mechanical Engineering, Johns Hopkins University , Baltimore, Maryland 21218, United States.
4
Laboratory of Biochemical Genetics and Metabolism, The Rockefeller University , New York, New York 10065, United States.
5
Department of Investigative Dermatology, The Rockefeller University , New York, New York 10065, United States.
6
Department of Healthcare Policy and Research, Weill Cornell Medical College , New York, New York 10065, United States.
7
Department of Pathology, Memorial Sloan Kettering Cancer Center , New York, New York 10065, United States.
8
Department of Surgery, Memorial Sloan Kettering Cancer Center , New York, New York 10065, United States.
9
Department of Medicine, Memorial Sloan Kettering Cancer Center , New York, New York 10065, United States.
10
Department of Oncology, Johns Hopkins University , Baltimore, Maryland 21218, United States.

Abstract

White adipose tissue inflammation (WATi) has been linked to the pathogenesis of obesity-related diseases, including type 2 diabetes, cardiovascular disease, and cancer. In addition to the obese, a substantial number of normal and overweight individuals harbor WATi, putting them at increased risk for disease. We report the first technique that has the potential to detect WATi noninvasively. Here, we used Raman spectroscopy to detect WATi with excellent accuracy in both murine and human tissues. This is a potentially significant advance over current histopathological techniques for the detection of WATi, which rely on tissue excision and, therefore, are not practical for assessing disease risk in the absence of other identifying factors. Importantly, we show that noninvasive Raman spectroscopy can diagnose WATi in mice. Taken together, these results demonstrate the potential of Raman spectroscopy to provide objective risk assessment for future cardiometabolic complications in both normal weight and overweight/obese individuals.

PMID:
26752499
PMCID:
PMC4799653
DOI:
10.1021/acs.analchem.5b03696
[Indexed for MEDLINE]
Free PMC Article

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