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Nat Genet. 2016 Feb;48(2):167-75. doi: 10.1038/ng.3481. Epub 2016 Jan 11.

Genomic analysis of 38 Legionella species identifies large and diverse effector repertoires.

Author information

1
Department of Cell Research and Immunology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.
2
Department of Microbiology, University of Chicago, Chicago, Illinois, USA.
3
Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.
4
Biology Division, Argonne National Laboratory and Department of Ecology and Evolution, University of Chicago, Chicago, Illinois, USA.

Abstract

Infection by the human pathogen Legionella pneumophila relies on the translocation of ∼ 300 virulence proteins, termed effectors, which manipulate host cell processes. However, almost no information exists regarding effectors in other Legionella pathogens. Here we sequenced, assembled and characterized the genomes of 38 Legionella species and predicted their effector repertoires using a previously validated machine learning approach. This analysis identified 5,885 predicted effectors. The effector repertoires of different Legionella species were found to be largely non-overlapping, and only seven core effectors were shared by all species studied. Species-specific effectors had atypically low GC content, suggesting exogenous acquisition, possibly from the natural protozoan hosts of these species. Furthermore, we detected numerous new conserved effector domains and discovered new domain combinations, which allowed the inference of as yet undescribed effector functions. The effector collection and network of domain architectures described here can serve as a roadmap for future studies of effector function and evolution.

PMID:
26752266
PMCID:
PMC5050043
DOI:
10.1038/ng.3481
[Indexed for MEDLINE]
Free PMC Article

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