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Nat Nanotechnol. 2016 Apr;11(4):388-94. doi: 10.1038/nnano.2015.312. Epub 2016 Jan 11.

Bioinspired fluorescent dipeptide nanoparticles for targeted cancer cell imaging and real-time monitoring of drug release.

Fan Z1,2, Sun L1,2, Huang Y1,2, Wang Y1,2, Zhang M1,2,3.

Author information

1
Department of Biomedical Engineering, College of Engineering, The Ohio State University, Columbus, Ohio 43210, USA.
2
Dorothy M. Davis Heart &Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, Ohio 43210, USA.
3
Interdisciplinary Biophysics Graduate Program, The Ohio State University, Columbus, Ohio 43210, USA.

Abstract

Peptide nanostructures are biodegradable and are suitable for many biomedical applications. However, to be useful imaging probes, the limited intrinsic optical properties of peptides must be overcome. Here we show the formation of tryptophan-phenylalanine dipeptide nanoparticles (DNPs) that can shift the peptide's intrinsic fluorescent signal from the ultraviolet to the visible range. The visible emission signal allows the DNPs to act as imaging and sensing probes. The peptide design is inspired by the red shift seen in the yellow fluorescent protein that results from π-π stacking and by the enhanced fluorescence intensity seen in the green fluorescent protein mutant, BFPms1, which results from the structure rigidification by Zn(II). We show that DNPs are photostable, biocompatible and have a narrow emission bandwidth and visible fluorescence properties. DNPs functionalized with the MUC1 aptamer and doxorubicin can target cancer cells and can be used to image and monitor drug release in real time.

PMID:
26751169
DOI:
10.1038/nnano.2015.312
[Indexed for MEDLINE]

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